4-Nonylphenol triggers apoptosis and affects 17-β-Estradiol receptors in calvarial osteoblasts

被引：22
作者：
Sabbieti, Maria Giovanna [1 ]
Agas, Dimitrios [1 ]
Palermo, Francesco [1 ]
Mosconi, Gilberto [1 ]
Santoni, Giorgio [2 ]
Amantini, Consuelo [2 ]
Farfariello, Valerio [2 ]
Marchetti, Luigi [1 ]
机构：
[1] Univ Camerino, Sch Biosci & Biotechnol, I-62032 Camerino, MC, Italy
[2] Univ Camerino, Sch Pharm, I-62032 Camerino, MC, Italy
来源：
TOXICOLOGY | 2011年 / 290卷 / 2-3期
关键词：
Alkylphenols; Osteoblasts; Apoptosis; MOUSE BONE-MARROW; ESTROGEN-RECEPTOR; ENVIRONMENTAL ESTROGENS; STROMAL CELLS; DIFFERENTIATION; PROLIFERATION; NONYLPHENOL; EXPRESSION; ALPHA; GENE;
D O I：
10.1016/j.tox.2011.10.014
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The present research examines the effects of 4-nonylphenol (4-NP) on mouse primary calvarial osteoblasts(COBs). Incubation of the cells with 4-NP at 10(-5) M and 10(-6) M striking decreased osteoblasts viability and phosphatidylserine (PS) exposure, measured by Annexin V. was greatly enhanced. In addition, an up-regulation of Bax/Bcl2 ratio with a drop in Delta Psi m and an increase of cleaved caspase 9 and 3 was found, suggesting that the alkylphenol induced osteoblast death via the mitochondrial-dependent apoptotic pathway. Interestingly, treatment with 4-NP was also able to increase cleaved caspase 8 in parallel with the truncated active Bid (t-Bid) suggesting that 4-NP-mediated apoptosis depends on cross talk between the extrinsic and intrinsic pathways. It is of relevance, that the apoptotic effects of 4-NP overcame 17-beta-Estradiol (17-beta E-2) induced-survival on osteoblasts. Also, the alkylphenol interfered with 17-beta E-2 regulated estrogen receptors expression. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：334 / 341
页数：8
相关论文
共 56 条
[1]   17β-estradiol and environmental estrogens significantly affect mammalian sperm function [J].
Adeoya-Osiguwa, SA ;
Markoulaki, S ;
Pocock, V ;
Milligan, SR ;
Fraser, LR .
HUMAN REPRODUCTION, 2003, 18 (01) :100-107
[2]   Benzyl butyl phthalate influences actin distribution and cell proliferation in rat Py1a Osteoblasts [J].
Agas, Dimitrios ;
Sabbieti, Maria G. ;
Capacchietti, Mariolina ;
Materazzi, Stefano ;
Menghi, Giovanna ;
Materazzi, Giovanni ;
Hurley, Marja M. ;
Marchetti, Luigi .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 101 (03) :543-551
[3]   Triggering of transient receptor potential vanilloid type 1 (TRPV1) by capsaicin induces Fas/CD95-mediated apoptosis of urothelial cancer cells in an ATM-dependent manner [J].
Amantini, Consuelo ;
Ballarini, Patrizia ;
Caprodossi, Sara ;
Nabissi, Massimo ;
Morelli, Maria Beatrice ;
Lucciarini, Roberta ;
Cardarelli, Marco Andrea ;
Mammana, Gabriele ;
Santoni, Giorgio .
CARCINOGENESIS, 2009, 30 (08) :1320-1329
[4]   DEMONSTRATION OF ESTROGEN AND VITAMIN-D RECEPTORS IN BONE-MARROW-DERIVED STROMAL CELLS - UP-REGULATION OF THE ESTROGEN-RECEPTOR BY 1,25-DIHYDROXYVITAMIN-D(3) [J].
BELLIDO, T ;
GIRASOLE, G ;
PASSERI, G ;
YU, XP ;
MOCHARLA, H ;
JILKA, RL ;
NOTIDES, A ;
MANOLAGAS, SC .
ENDOCRINOLOGY, 1993, 133 (02) :553-562
[5]   FORMATION OF MINERALIZED NODULES BY BONE DERIVED CELLS-INVITRO - A MODEL OF BONE-FORMATION [J].
BERESFORD, JN ;
GRAVES, SE ;
SMOOTHY, CA .
AMERICAN JOURNAL OF MEDICAL GENETICS, 1993, 45 (02) :163-178
[6]   Deuterium labelled nonylphenols in an in-vitro model of percutaneous absorption of environmental xenoestrogens [J].
Blume, B ;
Kietzmann, M ;
Kränke, P ;
Möder, M ;
Schrader, S ;
Wahren, M .
ISOTOPES IN ENVIRONMENTAL AND HEALTH STUDIES, 2000, 36 (01) :3-9
[7]   Estrogen receptor-α is developmentally regulated during osteoblast differentiation and contributes lo selective responsiveness of gene expression [J].
Bodine, PVN ;
Henderson, RA ;
Green, J ;
Aronow, M ;
Owen, T ;
Stein, GS ;
Lian, JB ;
Komm, BS .
ENDOCRINOLOGY, 1998, 139 (04) :2048-2057
[8]   The effects of estrogen on osteoprotegerin, RANKL, and estrogen receptor expression in human osteoblasts [J].
Bord, S ;
Ireland, DC ;
Beavan, SR ;
Compston, JE .
BONE, 2003, 32 (02) :136-141
[9]   Localization of estrogen receptor β protein expression in adult human bone [J].
Braidman, IP ;
Hainey, L ;
Batra, G ;
Selby, PL ;
Saunders, PTK ;
Hoyland, JA .
JOURNAL OF BONE AND MINERAL RESEARCH, 2001, 16 (02) :214-220
[10]   The effects of 4-nonylphenol in rats: A multigeneration reproduction study [J].
Chapin, RE ;
Delaney, J ;
Wang, YF ;
Lanning, L ;
Davis, B ;
Collins, B ;
Mintz, N ;
Wolfe, G .
TOXICOLOGICAL SCIENCES, 1999, 52 (01) :80-91
123456