Electrospinning Gelatin/Chitosan/Hydroxyapatite/Graphene Oxide Composite Nanofibers with Antibacterial Properties

Gelatin/chitosan/hydroxyapatite/graphene oxide composite nanofibers were prepared by electrospinning. The effect of composition on fiber morphology and antibacterial properties were investigated. The results show that increasing of gelatin concentration results in the increase of fiber diameter and then heavy fiber adhesion, showing ideal concentration scope of 15%-20%. Increasing chitosan concentration leads to thin fibers, with optimum concentration at 1%, while increasing hydroxyapatite (HA) concentration increases the ionic concentration of eletrospinning solution, leading to the decrease of beads and fiber adhesion. Nanofibers with smooth morphology are obtained when the diameter of HA particles is 12 mu m and at concentration of 5%. And the addition of graphene oxide (GO) enhances uniform, smooth and antibacterial property of the composite nanofibers, which enables the gelatin/chitosan/hydroxyapatite/GO fibers good antibacterial effect against both Staphylococcus aureus and Escherichia coli.