Cloning and partial characterization of a β-1,3-glucanase gene from strawberry

被引：10

作者：

Khan, AA

Shi, Y

Shih, DS ^{[1
]}

机构：

[1] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA

[2] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA

来源：

DNA SEQUENCE | 2003年 / 14卷 / 06期

关键词：

beta-1,3-glucanase gene; pathogenesis-related proteins; strawberry (Fragaria x ananassa Duch); polymerase chain reaction; CLADOSPORIUM-FULVUM; TRANSGENIC TOBACCO; ELICITOR RESPONSE; PROTEINS; TOMATO; EXPRESSION; RESISTANCE; PLANTS; CHITINASES; SIGNAL;

D O I：

10.1080/10425170310001608399

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant pathogenesis-related proteins, including beta-1,3-glucanses, are believed to be involved in plant defense mechanisms. We have cloned a beta-1,3-glucanse gene from strawberry ( Fragaria x ananassa Duch). This gene, designated FaBG2-1 , is a Class II glucanase gene composed of two exons and one intron. The location of a 397-base intron in the gene was confirmed by sequencing a partial cDNA clone obtained by using a rapid amplification of cDNA ends procedure. Also, based on the cDNA sequence, the transcription start site of FaBG2-1 was assigned to a -54G residue. Genomic Southern blot analysis indicated that FaBG2-1 is a member of a multi-gene family. Reverse transcriptase-polymerase chain reaction analysis revealed that this beta-1,3-glucanase gene is expressed constitutively in strawberry leaves.

引用

收藏

页码：406 / 412

页数：7

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