Immune responses of chickens inoculated with a recombinant fowlpox vaccine coexpressing glycoprotein B of infectious laryngotracheitis virus and chicken IL-18

被引：19
作者：
Chen, Hong-Ying [1 ]
Cui, Pei [2 ]
Cui, Bao-An [1 ]
Li, He-Ping [1 ]
Jiao, Xian-Qin [1 ]
Zheng, Lan-Lan [1 ]
Cheng, Guo [2 ]
Chao, An-Jun [1 ]
机构：
[1] Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan Province, Peoples R China
[2] Anim Husb Bur Henan Prov, Henan Ctr Anim Dis Control & Prevent, Zhengzhou, Henan Province, Peoples R China
来源：
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2011年 / 63卷 / 02期
关键词：
chicken interleukin-18; gB gene; infectious laryngotracheitis virus; recombinant fowlpox virus; DISEASE VIRUS; DNA VACCINE; SWINE IL-18; INTERLEUKIN-18; PROTECTION; GENES; ENHANCEMENT; EFFICACY; TH1;
D O I：
10.1111/j.1574-695X.2011.00850.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes severe and economically significant respiratory disease in poultry worldwide. Herein, the immunogenicity of two recombinant fowlpox viruses (rFPV-gB and rFPV-gB/IL18) containing ILTV glycoprotein B (gB) and chicken interleukin-18 (IL-18) were investigated in a challenge model. One-day-old specific-pathogen-free chickens were vaccinated by wing-web puncture with the two rFPVs and challenged with the virulent ILTV CG strain. There were differences in antibody levels elicited by either rFPV-gB/IL18 or rFPV-gB as determined using ELISA. The ratios of CD4(+) to CD8(+) in chickens immunized with rFPV-gB/IL18 were higher (P < 0.05) than in those immunized with rFPV-gB, and the level of proliferative response of the T cells in the rFPV-gB/IL18-vaccinated group was higher (P < 0.05) than that in the rFPV-gB group. All chickens immunized with rFPV-gB/IL18 were protected (10/10), whereas only eight of 10 of the chickens immunized with the rFPV-gB were protected. The results showed that the protective efficacy of the rFPV-gB vaccine could be enhanced by simultaneous expression of chicken IL-18.
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收藏
页码：289 / 295
页数：7
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