Over-expression of miR-125a-5p inhibits proliferation in C2C12 myoblasts by targeting E2F3

被引：20

作者：

Song, Chengchuang ^{[1
]}

Wu, Guofang ^{[1
]}

Xiang, Aoqi ^{[1
]}

Zhang, Qiangling ^{[1
]}

Li, Wanhua ^{[1
]}

Yang, Gongshe ^{[1
]}

Shi, Xin'e ^{[1
]}

Sun, Shiduo ^{[1
]}

Li, Xiao ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Anim Sci & Technol, Lab Anim Fat Deposit & Muscle Dev, Yangling 712100, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2015年 / 47卷 / 04期

关键词：

microRNAs; miR-125a-5p; proliferation; E2F3; SKELETAL MYOGENESIS; CELL-GROWTH; DIFFERENTIATION; MICRORNAS; VERTEBRATES; APOPTOSIS; PATHWAY; CANCER; PLAYS;

D O I：

10.1093/abbs/gmv006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are a class of small non-coding RNAs of 20-25 nucleotides in length. It has been shown that miRNAs play important roles in the proliferation of many types of cells, including myoblasts. In this study, we used real-time quantitative polymerase chain reaction, western blotting, EdU, flow cytometry, and CCK-8 assay to explore the role of miR-125a-5p during the proliferation of C2C12 myoblasts. It was found that the expression of miR-125a-5p was decreased during C2C12 myoblast proliferation. Over-expression of miR-125a-5p inhibited C2C12 myoblast proliferation as indicated by EdU staining, flow cytometry, and CCK8 assay. It was also found that miR-125a-5p could negatively regulate E2F3 expression at posttranscriptional level, via a specific target site in the 30 untranslated region. Knockdown of E2F3 showed a similar inhibitory effect on C2C12 myoblast proliferation. Thus, our findings suggest that miR-125a-5p may act as a negative regulator of C2C12 myoblast proliferation by targeting E2F3.

引用

页码：244 / 249

页数：6

共 31 条

[1] E2f3b plays an essential role in myogenic differentiation through isoform-specific gene regulation [J].