Separation and determination of bases by capillary zone electrophoresis

被引：0

作者：

Zhao, T ^{[1
]}

Liu, QP ^{[1
]}

Cheng, JK ^{[1
]}

机构：

[1] WUHAN UNIV,DEPT CHEM,WUHAN 430072,PEOPLES R CHINA

来源：

CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 1997年 / 13卷 / 03期

关键词：

capillary zone electrophoresis; base; electrophoresis;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The present paper covers the separation and determination of cytosine, 9-N-dihydroxypropyltheophylline , theobromine, adenine, guanine, uracil, theophylline, 1-hydroxyethyl-5-fluorouracil, xanthine, uric acid, 6-mercaptopurine, 5-fluorouracil and 1-propionyloxyuracil by capillary zone electrophoresis. In an uncoated silica capillary(70 cm X 75 mu m i. d. effective length 63 cm), thirteen bases could be separated within 11 min under the optimum conditions: 0.01 mol/L(pH 10.0) borate buffer, 40 degrees C, 15 kV, 260 nm and 2 s load. Efficiencies up to 3.1 X 10(5) theoretical plates(49 X 10(5) plates/m) were achieved.

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页码：215 / 220

页数：6

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