Virulence variations of Flavobacterium columnare in rainbow trout (Oncorhynchus mykiss) eyed eggs and alevin

Flavobacterium columnare (Fc) is the causative agent for columnaris disease (CD) in several fish species and an emerging problem for rainbow trout aquaculture. We characterize the virulence phenotype of two Fc isolates, CSF-298-10 and MS-FC-4, against trout from two sources, NCCCWA and a production stock (PS), at the eyed egg and alevin life stages. Immersion challenges demonstrated that NCCCWA eyed eggs were susceptible to the Fc isolate MS-FC-4 (>97% mortality) but no mortality was observed against PS eyed eggs. The CSF-298-10 had little effect on any eyed eggs tested and was not highly virulent to any alevin till day six post-hatch, up to 38% for NCCCWA and similar to 80% PS alevin. The MS-FC-4 strain produced 80% mortality any day an immersion challenge occurred post-hatch. Significant difference in CFU counts was recorded between the Fc strains on 2 days post-hatch immersion challenges. Counts for the NCCCWA alevin were 4.4 x 10(3) CFU/ml(-1) and 1.8 x 10(6) CFU/ml(-1) for the CSF-298-10 strain and MSFC-4 strain, respectively, and for the PS alevin CSF-298-10 measured 9.9 x 10(1) CFU/ ml(-1) and 3.8 x 10(5) CFU/ml(-1) for MS-FC-4. These two Fc isolates present stark differences in virulence phenotypes to both eyed eggs and alevin and present an interesting model system for virulence kinetics and potentially alternative pathogenic pathways.