Identification of Compounds in Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Seed Hull Extracts That Inhibit Lipopolysaccharide-Induced Inflammation in RAW 264.7 Macrophages

被引：65

作者：

Huang, Din-Wen ^{[2
]}

Chung, Cheng-Pei ^{[2
]}

Kuo, Yueh-Hsiung ^{[1
,3
,5
]}

Lin, Yun-Lian ^{[4
]}

Chiang, Wenchang ^{[2
]}

机构：

[1] China Med Univ, Tsuzuki Inst Tradit Med, Coll Pharm, Taichung 404, Taiwan

[2] Natl Taiwan Univ, Coll Bioresources & Agr, Ctr Food & Biomol, Grad Inst Food Sci & Technol, Taipei 106, Taiwan

[3] Natl Taiwan Univ, Dept Chem, Taipei 106, Taiwan

[4] Natl Res Inst Chinese Med, Taipei 112, Taiwan

[5] Acad Sinica, Taipei 115, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 22期

关键词：

Adlay; anti-inflammatory; nitric oxide; inducible nitric oxide synthase; cyclooxygenase; 2; eriodictyol; ceramide; SIGNAL-TRANSDUCTION; PROTEIN-KINASE; NITRIC-OXIDE; CERAMIDE; PROGESTERONE; FLAVONOIDS; COMPONENTS; RELEASE; CELLS;

D O I：

10.1021/jf9028514

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We investigated the effects of adlay seed hull (AH) extracts on the lipopolysaccharide-induced inflammatory response in RAW 264.7 macrophages. An AH ethanol extract (AHE) was partitioned into ethyl acetate, n-butanol, and water fractions. Silica gel chromatography of the ethyl acetate fraction yielded 15 subfractions: AHE-Ea-A to AHE-Ea-O. Subfractions AHE-Ea-J, AHE-Ea-K, and AHE-Ea-M had anti-inflammatory activities, as they counteracted the increased cellular production of nitric oxide and prostaglandin E2 induced by lipopolysaccharide by down-regulating inducible nitric oxide synthase and cyclooxygenase 2 expression. Eriodictyol (1), the ceramide (2S,3S,4R)-2-[(2'R)-2'-hydroxytetracosanoyl-amino]-1,3,4-octadecanetriol (2), and p-coumaric acid (3) were found in the subfractions, and the first two compounds appeared to be primarily responsible for the anti-inflammatory activity. This is the first time that eriodictyol (1) and this ceramide (2) have been found in AH, and the anti-inflammatory properties of the AHE-Ea fraction can be attributed, at least in part, to the presence of these two compounds.

引用

页码：10651 / 10657

页数：7

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