Prolyl-4-hydroxylase (AtP4H1) mediates and mimics low oxygen response in Arabidopsis thaliana

被引：30

作者：

Asif, Mehar Hasan ^{[1
]}

Trivedi, Prabodh Kumar ^{[1
]}

Misra, Prashant ^{[1
]}

Nath, Pravendra ^{[1
]}

机构：

[1] CSIR, Natl Bot Res Inst, Plant Gene Express Lab, Lucknow 226001, Uttar Pradesh, India

来源：

FUNCTIONAL & INTEGRATIVE GENOMICS | 2009年 / 9卷 / 04期

关键词：

Anoxia; AtP4H1; Hypoxia; Prolyl-4-hydroxylase; HYPOXIA-INDUCIBLE-FACTOR; PROLYL; 4-HYDROXYLASE; ARABINOGALACTAN PROTEIN; PHASEOLUS-VULGARIS; HYDROXYLASE; FAMILY; EXPRESSION; GENE; HIF; LOCALIZATION;

D O I：

10.1007/s10142-009-0118-y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Proline hydroxylation is an important phenomenon of a living cell. Prolyl-4-hydroxylases (P4H) responsible for this process have been characterized from animals, and one of its forms, HIF-P4H, is regarded as an oxygen sensor. In plants, P4H has been partially characterized from few species, and one of the Arabidopsis P4H (AtP4H1) has been shown to hydroxylate proline-rich peptides in vitro. In order to study its function in planta, we have overexpressed AtP4H1 in Arabidopsis. The AtP4H1oexp plants showed hypoxia-in-normoxia phenotype with strict requirement for carbon source for its growth, increased root hair, absence of trichome, and reduction in seed size. Genome-wide expression analyses suggest that expression of several genes related to hypoxia as well as plant growth and development are upregulated in AtP4H1oexp lines. Based on our studies on AtP4H1oexp lines, we speculate a direct role of AtP4H1 in hypoxia stress and in different stages of plant growth and development.

引用

页码：525 / 535

页数：11

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