In vitro and in vivo characterisation of Listeria monocytogenes outbreak isolates

被引:19
作者
Stratakos, Alexandros Ch [1 ,3 ,8 ]
Ijaz, Umer Zeeshan [2 ]
Ward, Patrick [3 ]
Linton, Mark [1 ]
Kelly, Carmel [1 ]
Pinkerton, Laurette [1 ]
Scates, Pam [1 ]
McBride, Jane [1 ]
Pet, Ioan [4 ]
Criste, Adriana [5 ]
Stef, Ducu [6 ]
Couto, Jillian M. [2 ]
Sloan, William T. [2 ]
Dorrell, Nick [7 ]
Wren, Brendan W. [7 ]
Stef, Lavinia [4 ]
Gundogdu, Ozan [7 ]
Corcionivoschi, Nicolae [1 ,4 ,5 ]
机构
[1] Agrifood & Biosci Inst, Bacteriol Branch, Vet Sci Div, Belfast, Antrim, North Ireland
[2] Univ Glasgow, Sch Engn, Glasgow G12 8LT, Lanark, Scotland
[3] NovaUCD, Auranta, Dublin, Ireland
[4] Banat Univ Anim Sci & Vet Med King Michael I Roma, Sch Bioengn Anim Sci Resources, Timisoara, Romania
[5] Univ Anim Sci & Vet Med, Sch Anim Sci & Biotechnol, Cluj Napoca, Romania
[6] Banat Univ Anim Sci & Vet Med King Michael 1 Roma, Fac Food Engn, Timisoara, Romania
[7] London Sch Hyg & Trop Med, Fac Infect & Trop Dis, 11 Keppel St, London WC1E 7HT, England
[8] Univ West England UWE Bristol, Bristol BS16 1QY, Avon, England
关键词
Listeria monocytogenes; Outbreaks; Environment; Genomics; Virulence; STRESS-RESPONSE; GENE-EXPRESSION; READ ALIGNMENT; ACID STRESS; VIRULENCE; INFECTION; VISUALIZATION; TOLERANCE; SURVIVAL; INNOCUA;
D O I
10.1016/j.foodcont.2019.106784
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Listeriosis is an important food-borne disease responsible for high rates of morbidity and mortality. L. monocytogenes has been the cause of several foodborne outbreaks and its ability to adapt and survive in a wide range of environmental conditions makes eradication difficult. Many L. monocytogenes strains are avirulent but have the ability to increase their virulence if exposed to environmental stresses. The aim of this study was to explain the observed increase in virulence of outbreak L. monocytogenes isolates by using phenotypic assays and whole genome sequencing. Four L. monocytogenes isolates from sweetcorn and one isolate from a raw milk (control) were sequenced and characterised using a range of phenotypic assays. The four L. monocytogenes sweetcorn isolates displayed a significant increase for in vitro adhesion and invasion of epithelial cells compared to the control isolate. They also showed a higher level of colonisation of the liver and spleen in vivo. In addition, the four L. monocytogenes isolates displayed an increased ability to form biofilms, resist heat stress and resist a combination of antimicrobials. Investigation of the genomes of the four L. monocytogenes sweet corn isolates identified Single Nucleotide Polymorphisms (SNPs) in genes, which may have a role in the observed phenotypes characteristic of these strains, particularly in response to survival properties within the environment or in terms of virulence. We highlight the importance of combining whole genomic sequencing with phenotypic characterisation as a key element in the investigation of outbreaks of foodborne pathogens.
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页数:10
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