Protein phosphatase AP2C1 negatively regulates basal resistance and defense responses to Pseudomonas syringae

被引：42

作者：

Shubchynskyy, Volodymyr ^{[1
,2
]}

Boniecka, Justyna ^{[1
,2
]}

Schweighofer, Alois ^{[1
,2
,3
]}

Simulis, Justinas ^{[3
]}

Kvederaviciute, Kotryna ^{[3
]}

Stumpe, Michael ^{[4
]}

Mauch, Felix ^{[4
]}

Balazadeh, Salma ^{[5
,6
]}

Mueller-Roeber, Bernd ^{[5
,6
]}

Boutrot, Freddy ^{[7
]}

Zipfel, Cyril ^{[7
]}

Meskiene, Irute ^{[1
,2
,3
,8
]}

机构：

[1] Univ Vienna, Max F Perutz Labs, Dr Bohrgasse 9, A-1030 Vienna, Austria

[2] Med Univ Vienna, Dr Bohrgasse 9, A-1030 Vienna, Austria

[3] Univ Vilnius, Inst Biotechnol IBT, Sauletekio Al 7, LT-10257 Vilnius, Lithuania

[4] Dept Biol, Chemin Musee 10, CH-1700 Fribourg, Switzerland

[5] Max Planck Inst Mol Plant Physiol, Golm, Germany

[6] Univ Potsdam, D-14476 Golm, Germany

[7] Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[8] Univ Vienna, Dept Ecogen & Syst Biol, Althanstr 14, A-1090 Vienna, Austria

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2017年 / 68卷 / 05期

基金：

欧洲研究理事会; 英国生物技术与生命科学研究理事会; 奥地利科学基金会;

关键词：

Callose; defense genes; MAPK; MAPK phosphatase; PAMP; PP2C phosphatase; Pseudomonas syringae; salicylic acid; transcription factors; SYSTEMIC ACQUIRED-RESISTANCE; ARABIDOPSIS INNATE IMMUNITY; TRANSCRIPTION FACTORS; DISEASE RESISTANCE; GENE-EXPRESSION; PLANT IMMUNITY; SALICYLIC-ACID; ETHYLENE BIOSYNTHESIS; CALLOSE DEPOSITION; BOTRYTIS-CINEREA;

D O I：

10.1093/jxb/erw485

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Mitogen-activated protein kinases (MAPKs) mediate plant immune responses to pathogenic bacteria. However, less is known about the cell autonomous negative regulatory mechanism controlling basal plant immunity. We report the biological role of Arabidopsis thaliana MAPK phosphatase AP2C1 as a negative regulator of plant basal resistance and defense responses to Pseudomonas syringae. AP2C2, a closely related MAPK phosphatase, also negatively controls plant resistance. Loss of AP2C1 leads to enhanced pathogen-induced MAPK activities, increased callose deposition in response to pathogen-associated molecular patterns or to P. syringae pv. tomato (Pto) DC3000, and enhanced resistance to bacterial infection with Pto. We also reveal the impact of AP2C1 on the global transcriptional reprogramming of transcription factors during Pto infection. Importantly, ap2c1 plants show salicylic acid-independent transcriptional reprogramming of several defense genes and enhanced ethylene production in response to Pto. This study pinpoints the specificity of MAPK regulation by the different MAPK phosphatases AP2C1 and MKP1, which control the same MAPK substrates, nevertheless leading to different downstream events. We suggest that precise and specific control of defined MAPKs by MAPK phosphatases during plant challenge with pathogenic bacteria can strongly influence plant resistance.

引用

页码：1169 / 1183

页数：15

共 81 条

[1] Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae [J].