AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study

被引：173

作者：

Ruozi, Barbara ^{[1
]}

Belletti, Daniela ^{[1
]}

Tombesi, Andrea ^{[2
]}

Tosi, Giovanni ^{[1
]}

Bondioli, Lucia ^{[1
]}

Forni, Flavio ^{[1
]}

Vandelli, Maria Angela ^{[1
]}

机构：

[1] Univ Modena & Reggio Emilia, Dept Pharmaceut Sci, Modena, Italy

[2] Univ Modena & Reggio Emilia, CIGS, Modena, Italy

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2011年 / 6卷

关键词：

atomic force microscopy; transmission electron microscopy; environmental scanning electron microscopy; confocal laser scanning microscopy; ATOMIC-FORCE MICROSCOPY; FREEZE-FRACTURE; CRYOELECTRON MICROSCOPY; SIZE DISTRIBUTION; SUSPENSIONS; MEMBRANES; DRUG; TOOL;

D O I：

10.2147/IJN.S14615

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

An outstanding aspect of pharmaceutical nanotechnology lies in the characterization of nanocarriers for targeting of drugs and other bioactive agents. The development of microscopic techniques has made the study of the surface and systems architecture more attractive. In the field of pharmaceutical nanosystems, researchers have collected vital information on size, stability, and bilayer organization through the microscopic characterization of liposomes. This paper aims to compare the results obtained by atomic force microscopy, environmental scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy to point out the limits and advantages of these applications in the evaluation of vesicular systems. Besides this comparative aim, our work proposes a simple confocal laser scanning microscopy procedure to rapidly and easily detect the liposomal membrane.

引用

页码：557 / 563

页数：7

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