Use of Brucella abortus species specific polymerase chain reaction assay for the diagnosis of bovine brucellosis

被引：4
作者：
Chisi, Songelwayo L. [1 ]
Schmidt, Tracy [1 ]
Akol, George W. [2 ,3 ]
van Heerden, Henriette [4 ]
机构：
[1] Dept Agr & Rural Dev KwaZulu Natal Prov, Allerton Prov Vet Lab, Pietermaritzburg, South Africa
[2] Dohne Agr Dev Inst, Ctr Vet Excellence, Stutterheim, South Africa
[3] Dept Rural Dev & Agrarian Reform, Stutterheim, South Africa
[4] Univ Pretoria, Dept Trop Dis, Pretoria, South Africa
来源：
JOURNAL OF THE SOUTH AFRICAN VETERINARY ASSOCIATION | 2017年 / 88卷
关键词：
PCR; FETUSES; BLOOD; SHEEP; DNA;
D O I：
10.4102/jsava.v88i0.1433
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Serology is primarily used in the diagnosis of bovine brucellosis. Bacterial culture and isolation is the gold standard in diagnosing brucellosis but, like serology, it does not offer complete (100%) diagnostic sensitivity and specificity. Polymerase chain reaction (PCR) has been suggested to offer better specificity and sensitivity. In this study, we evaluated the performance of Brucella abortus species specific (BaSS) PCR directly from different samples in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture. The BaSS PCR had a low diagnostic sensitivity (DSe) of 70%, but was able to identify vaccine strains using abomasal fluid from aborted foetuses and detect Brucella DNA from decomposing samples. The best sample for the BaSS PCR was abomasal fluid.
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页数：3
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