A novel iron-regulated metal transporter from plants identified by functional expression in yeast

被引：1074

作者：

Eide, D ^{[1
]}

Broderius, M ^{[1
]}

Fett, J ^{[1
]}

Guerinot, ML ^{[1
]}

机构：

[1] DARTMOUTH COLL,DEPT BIOL SCI,HANOVER,NH 03755

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 11期

关键词：

Fe(II) transport; iron deficiency; strategy I response;

D O I：

10.1073/pnas.93.11.5624

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Iron is an essential nutrient for virtually all organisms. The IRT1 (iron-regulated transporter) gene of the plant Arabidopsis thaliana, encoding a probable Fe(II) transporter, was cloned by functional expression in a yeast strain defective for iron uptake. Yeast expressing IRT1 possess a novel Fe(II) uptake activity that is strongly inhibited by Cd. IRT1 is predicted to be an integral membrane protein with a metal-binding domain. Data base comparisons and Southern blot analysis indicated that IRT1 is a member of a gene family in Arabidopsis. Related sequences were also found in the genomes of rice, yeast, nematodes, and humans. In Arabidopsis, IRT1 is expressed in roots, is induced by iron deficiency, and has altered regulation in plant lines bearing mutations that affect the iron uptake system. These results provide the first molecular insight into iron transport by plants.

引用

页码：5624 / 5628

页数：5

共 39 条

[21] METALLOENZYMES, STRUCTURAL MOTIFS, AND INORGANIC MODELS [J].