phbB, phbC gene expression in E-coli and their transformation and identification in potato

Using NADPH-dependent acetoacetyl-CoA reductase gene (phbB) and poly-beta-hydroxybutyrate (PHB) synthase gene (phbC) cloned from Alcaligenes eutrophus H16 and expression vector pKK223-3, the authors constructed an E. coli expression vector pKCB containing independent phbB and phbC operators, respectively, and transfered it into E. coli JM109. The microscopy and GC analysis indicated that E. coli JM109 (containing pKCB) induced by IPTG could synthesize poly-beta-hydroxybutyrate (PHB). By DNA processing, three tuber-specific plant expression vectors, pP SAGE (containing phbB), pBIBGC (containing phbC) and pPSAGCB (containing both phbB and phbC), were successfully constructed. In 5 transformed potato cultivars, the authors screened 20 positive lines.