The synthesis of temperature-sensitive molecularly imprinted film on support beads and its application for bovine serum albumin separation

A new type of protein molecularly imprinted microspheres (MIMPs) with both temperature-sensitive and magnetic was prepared for selective separation of template protein. The microspheres were obtained via precipitation polymerization method in the presence of temperature-sensitive monomer N-isopropylacrylamide (NIPAM), functional monomer methacrylic acid (MAA) and cross-linking agent N,N'-methylenebisacrylamide (MBA). Importantly, the MIMPs were composed of a core Fe3O4 bead, a sandwiched layer of poly(4-vinyl pyridine) (4-VP) and a shell of crosslinked poly(N-isopropylacrylamide) (PNIPAM). Pyridyl ring on the magnetic beads was hydrophilic group which could benefit the dispersion in water and the capture of the template bovine serum albumin (BSA). The MIMPs were investigated by transmission electron microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Adsorption experiments and practical analytical performance demonstrated that the MIMPs exhibited excellent binding properties, including high adsorption capacity (79.2 mg/g), specific recognition, a prevalent preference for bovine serum albumin (BSA), and a favorable reusability. Furthermore, the influence of the external temperature on the adsorption and desorption was also investigated, and the property exhibited its potential application in rapid capture and separation of BSA. Therefore, the prepared MIMPs are suitable for separation and provide a significant reference for other proteins in proteomics. (C) 2016 Elsevier B.V. All rights reserved.