The Phosphorylation of Lipid Transfer Protein CaMBP10

被引：6

作者：

Li, Cuifeng ^{[1
]}

Xie, Wanqin ^{[1
]}

Wang, Liqiang ^{[1
]}

Zhao, Yulong ^{[1
]}

机构：

[1] Nankai Univ, Dept Biochem & Mol Biol, Tianjin 300071, Peoples R China

来源：

PROTEIN AND PEPTIDE LETTERS | 2011年 / 18卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Calcium-dependent protein kinase; calmodulin-binding protein-10; calmodulin; lipid transfer protein; phosphorylation; CALMODULIN-BINDING PROTEIN; IDENTIFICATION;

D O I：

10.2174/092986611794328681

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calmodulin-binding protein-10 (CaMBP10) was isolated previously from Chinese cabbage and identified as a member of the lipid transfer protein family. In this study, we found that CaMBP10 was phosphorylated in a calcium(Ca2+)-dependent manner, and the phosphorylation was inhibited by calmodulin (CaM) antagonists. In-gel kinase assay revealed that the phosphorylation of CaMBP10 was catalyzed by a 45 kDa protein kinase, which underwent autophosphorylation in the presence of Ca2+. Immunoblotting assay further identified this kinase as a calcium-dependent protein kinase (CDPK). In addition, the phosphorylation site was mapped to the C-terminal region of CaMBP10, where the CaM-binding domain resides. These results provide novel insights into the molecular mechanisms that regulate CaMBP10 functions.

引用

页码：17 / 22

页数：6

共 28 条

[1] Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase [J].

Bachmann, M ;

Shiraishi, N ;

Campbell, WH ;

Yoo, BC ;

Harmon, AC ;

Huber, SC .

PLANT CELL, 1996, 8 (03) :505-517

[2] From elicitins to lipid-transfer proteins:: a new insight in cell signalling involved in plant defence mechanisms [J].

Blein, JP ;

Coutos-Thévenot, P ;

Marion, D ;

Ponchet, M .

TRENDS IN PLANT SCIENCE, 2002, 7 (07) :293-296

[3] Plant-specific calmodulin-binding proteins [J].

Bouché, N ;

Yellin, A ;

Snedden, WA ;

Fromm, H .

ANNUAL REVIEW OF PLANT BIOLOGY, 2005, 56 :435-466

[4] A lipid transfer protein binds to a receptor involved in the control of plant defence responses [J].

Buhot, N ;

Douliez, JP ;

Jacquemard, A ;

Marion, D ;

Tran, V ;

Maume, BF ;

Milat, ML ;

Ponchet, M ;

Mikès, V ;

Kader, JC ;

Blein, JP .

FEBS LETTERS, 2001, 509 (01) :27-30

[5] Role of plant lipid transfer proteins in plant cell physiology - A concise review [J].

Carvalho, Andre de Oliveira ;

Gomes, Valdirene Moreira .

PEPTIDES, 2007, 28 (05) :1144-1153

[6] Binding mechanism of nonspecific lipid transfer proteins and their role in plant defense [J].

Cheng, CS ;

Samuel, D ;

Liu, YJ ;

Shyu, JC ;

Lai, SM ;

Lin, KF ;

Lyu, PC .

BIOCHEMISTRY, 2004, 43 (43) :13628-13636

[7] Accessibility of tobacco lipid transfer protein cavity revealed by 15N NMR relaxation studies and molecular dynamics simulations [J].

Da Silva, Pedro ;

Landon, Celine ;

Beltoise, Romain ;

Ponchet, Michel ;

Vovelle, Francoise .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2006, 64 (01) :124-132

[8]

DAI LX, 2000, ACTA SCI NAT U NANKA, V33, P126

[9] Circular dichroism and H-1 nuclear magnetic resonance studies on the solution and membrane structures of GAP-43 calmodulin-binding domain [J].

Hayashi, N ;

Matsubara, M ;

Titani, K ;

Taniguchi, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (12) :7639-7645

[10]

K, 1996, PLANT MOL BIOL, V47, P627

← 1 2 3 →