Respiratory nitrate ammonification by Shewanella oneidensis MR-1

被引：100

作者：

Cruz-Garcia, Claribel

Murray, Alison E.

Klappenbach, Joel A.

Stewart, Valley

Tiedje, James M.

机构：

[1] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA

[2] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA

[3] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

[4] Univ Calif Davis, Microbiol Sect, Davis, CA 95616 USA

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 02期

关键词：

D O I：

10.1128/JB.01194-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.

引用

页码：656 / 662

页数：7

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