Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

被引：32

作者：

Zhao, Shu-Zhen ^{[1
]}

Sun, Hong-Zhao ^{[1
,2
]}

Gao, Ye ^{[1
]}

Sui, Na ^{[1
]}

Wang, Bao-Shan ^{[1
]}

机构：

[1] Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Peoples R China

[2] Shandong Inst Educ, Dept Biol Sci & Technol, Jinan 250013, Peoples R China

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2011年 / 47卷 / 03期

基金：

高等学校博士学科点专项科研基金;

关键词：

Betacyanin; Callus; DODA; Growth regulators; Suaeda salsa; H+-ATPASE ACTIVITY; SUSPENSION-CULTURES; CELL; INDUCTION; LIGHT; BETALAINS; EVOLUTION; SALINITY; ACID;

D O I：

10.1007/s11627-011-9339-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Suaeda salsa calluses cultured in darkness for 28 d were transferred to Murashige and Skoog (MS) media containing various growth regulators under white light conditions for 10 d to investigate cell growth, betacyanin accumulation, and expression of dopa-4,5-dioxygenase (DODA). Callus growth was markedly promoted when 0.2 mg center dot L-1 2,4-D and 0.5 mg center dot L-1 6-BA were added to the MS medium. Surprisingly, of the auxins tested, IAA had no effect on betacyanin content, but 2,4-D strongly decreased betacyanin content. Betacyanin content was positively correlated with 6-BA concentrations in the range of 0.1-2.0 mg center dot L-1. DODA mRNA levels were consistent with the response of betacyanin content to exogenous growth regulators. These results suggest that betacyanin metabolism in S. salsa calluses is regulated under white light conditions by growth regulators through the regulation of genes such as DODA that are involved in betacyanin synthesis.

引用

页码：391 / 398

页数：8