Strontium-induced repetitive calcium spikes in a unicellular green alga

被引:48
作者
Bauer, CS
Plieth, C
Bethmann, B
Popescu, O
Hansen, UP
Simonis, W
Schönknecht, G
机构
[1] Univ Wurzburg, D-97082 Wurzburg, Germany
[2] Univ Kiel, Inst Appl Phys, D-24098 Kiel, Germany
关键词
D O I
10.1104/pp.117.2.545
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The divalent cation Sr2+ induced repetitive transient spikes of the cytosolic Ca2+ activity [Ca2+](cy) and parallel repetitive transient hyperpolarizations of the plasma membrane in the unicellular green alga Eremosphaera viridis. [Ca2+](cy) measurements, membrane potential measurements, and cation analysis of the cells were used to elucidate the mechanism of Sr2+-induced [Ca2+](cy) oscillations. Sr2+ was effectively and rapidly compartmentalized within the cell, probably into the vacuole. The [Ca2+](cy) oscillations cause membrane potential oscillations, and not the reverse. The endoplasmic reticulum (ER) Ca2+-ATPase blockers 2,5-di-tert-butylhydroquinone and cyclopiazonic acid inhibited Sr2+-induced repetitive [Ca2+](cy) spikes, whereas the compartmentalization of Sr2+ was not influenced. A repetitive Ca2+ release and Ca2+ re-uptake by the ER probably generated repetitive [Ca-2+](cy) spikes in E. viridis in the presence of Sr2+. The inhibitory effect of ruthenium red and ryanodine indicated that the Sr2+-induced Ca2+ release from the ER was mediated by a ryanodine/cyclic ADP-ribose type of Ca2+ channel. The blockage of Sr2+-induced repetitive [Ca2+](cy) spikes by La3+ or Gd3+ indicated the necessity of a certain influx of divalent cations for sustained [Ca2+](cy) oscillations. Based on these data we present a mathematical model that describes the baseline spiking [Ca2+](cy) oscillations in E. viridis.
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收藏
页码:545 / 557
页数:13
相关论文
共 70 条
[1]   RELEASE OF CA2+ FROM INDIVIDUAL PLANT VACUOLES BY BOTH INSP(3) AND CYCLIC ADP-RIBOSE [J].
ALLEN, GJ ;
MUIR, SR ;
SANDERS, D .
SCIENCE, 1995, 268 (5211) :735-737
[2]   Repetitive Ca2+ spikes in a unicellular green alga [J].
Bauer, CS ;
Plieth, C ;
Hansen, UP ;
Sattelmacher, B ;
Simonis, W ;
Schonknecht, G .
FEBS LETTERS, 1997, 405 (03) :390-393
[3]   INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].
BERRIDGE, MJ .
NATURE, 1993, 361 (6410) :315-325
[4]   The AM and FM of calcium signalling [J].
Berridge, MJ .
NATURE, 1997, 386 (6627) :759-760
[5]   ELECTROCHEMICAL POTENTIAL GRADIENTS OF H+, K+, CA2+, AND CL- ACROSS THE TONOPLAST OF THE GREEN-ALGA EREMOSPHAERA-VIRIDIS [J].
BETHMANN, B ;
THALER, M ;
SIMONIS, W ;
SCHONKNECHT, G .
PLANT PHYSIOLOGY, 1995, 109 (04) :1317-1326
[6]   THE ELEMENTAL PRINCIPLES OF CALCIUM SIGNALING [J].
BOOTMAN, MD ;
BERRIDGE, MJ .
CELL, 1995, 83 (05) :675-678
[7]   CALCIUM OSCILLATIONS AND PROTEIN-SYNTHESIS INHIBITION SYNERGISTICALLY ACTIVATE MOUSE OOCYTES [J].
BOSMIKICH, A ;
SWANN, K ;
WHITTINGHAM, DG .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1995, 41 (01) :84-90
[8]   MEMBRANE TRAFFIC FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-APPARATUS IS DISTURBED BY AN INHIBITOR OF THE CA2+-ATPASE IN THE ER [J].
BUSCH, MB ;
SIEVERS, A .
PROTOPLASMA, 1993, 177 (1-2) :23-31
[9]   CALCIUM REGULATION IN PLANT-CELLS AND ITS ROLE IN SIGNALING [J].
BUSH, DS .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1995, 46 :95-122
[10]   CALCIUM SIGNALING [J].
CLAPHAM, DE .
CELL, 1995, 80 (02) :259-268