Birthweight determines intestinal microvasculature development and alters endothelial nitric oxide synthase density in young piglets

被引:2
作者
Ayuso, Miriam [1 ]
Van Cruchten, Steven [1 ]
Van Ginneken, Chris [1 ]
机构
[1] Univ Antwerp, Fac Biomed Pharmaceut & Vet Sci, Dept Vet Sci, Lab Appl Vet Morphol, Antwerp, Belgium
关键词
eNOS; intestinal microvasculature; low birth weight; pig; vWF; INTRAUTERINE GROWTH-RETARDATION; NECROTIZING ENTEROCOLITIS; BLOOD-FLOW; RESPONSES; MODEL; ENOS; CONSEQUENCES; HYPOXIA; PIG; AGE;
D O I
10.1111/ahe.12534
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Blood supply to enterocytes dictates intestinal health and nutrient absorption. These two aspects are impaired in low birthweight (LBW) piglets, but whether the perfusion to intestinal tissues is implicated as well is still unknown. Thus, structural changes in the microvasculature of LBW and normal birthweight (NBW) piglets were investigated during early postnatal development. Additionally, the presence of endothelial nitric oxide synthase (eNOS) in the intestinal mucosa was assessed given its important role to assure perfusion. A total of 22 pigs (11 LBW and 11 NBW) were sacrificed at days 0, 3, 8 and 19 of life. Body weight and intestinal length were recorded and a piece of the small intestine was sampled for immunohistochemical analysis of von Willebrand Factor (vWF, an endothelial cell marker) and eNOS. LBW piglets had a relatively (to body weight) longer intestine than their NBW counterparts. Age did not affect microvasculature, which was more abundant (85% larger vWF-positive area) in NBW than LBW pigs. However, an interaction age*BW was observed for eNOS-IR, showing that eNOS presence peaked in NBW piglets on the first day of life and subsequently decreased. This pattern was not observed in LBW piglets. The less abundant intestinal endothelial mass and the different pattern of eNOS expression observed in LBW piglets suggests microcirculation as a contributing factor in the impaired digestive functioning and gut health of LBW pigs. However, revealing whether the origin of this alteration is prenatal or postnatal, for example due to a lower milk intake, needs further study.
引用
收藏
页码:627 / 634
页数:8
相关论文
共 42 条
[1]   Intrauterine growth restricted piglets defined by their head shape ingest insufficient amounts of colostrum [J].
Amdi, C. ;
Krogh, U. ;
Flummer, C. ;
Oksbjerg, N. ;
Hansen, C. F. ;
Theil, P. K. .
JOURNAL OF ANIMAL SCIENCE, 2013, 91 (12) :5605-5613
[2]  
Ashworth CJ, 2001, J REP FER S, P233
[3]   Intestinal microcirculation and necrotizing enterocolitis: The vascular endothelial growth factor system [J].
Bowker, Rakhee M. ;
Yan, Xiaocai ;
De Plaen, Isabelle G. .
SEMINARS IN FETAL & NEONATAL MEDICINE, 2018, 23 (06) :411-415
[4]   Dimethyloxalylglycine preserves the intestinal microvasculature and protects against intestinal injury in a neonatal mouse NEC model: role of VEGF signaling [J].
Bowker, Rakhee M. ;
Yan, Xiaocai ;
Managlia, Elizabeth ;
Liu, Shirley X. L. ;
Marek, Catherine ;
Tan, Xiao-Di ;
De Plaen, Isabelle G. .
PEDIATRIC RESEARCH, 2018, 83 (02) :545-553
[5]   Prenatal gastrointestinal development in the pig and responses after preterm birth [J].
Buddington, R. K. ;
Sangild, P. T. ;
Hance, B. ;
Huang, E. Y. ;
Black, D. D. .
JOURNAL OF ANIMAL SCIENCE, 2012, 90 :290-298
[6]   How much formalin is enough to fix tissues? [J].
Buesa, Rene J. ;
Peshkov, Maxim V. .
ANNALS OF DIAGNOSTIC PATHOLOGY, 2012, 16 (03) :202-209
[7]   IUGR Does Not Predispose to Necrotizing Enterocolitis or Compromise Postnatal Intestinal Adaptation in Preterm Pigs [J].
Che, Lianqiang ;
Thymann, Thomas ;
Bering, Stine B. ;
Le Huerou-Luron, Isabelle ;
D'Inca, Romain ;
Zhang, Keying ;
Sangild, Per T. .
PEDIATRIC RESEARCH, 2010, 67 (01) :54-59
[8]   Morphogenesis and maturation of the embryonic and postnatal intestine [J].
Chin, Alana M. ;
Hill, David R. ;
Aurora, Megan ;
Spence, Jason R. .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2017, 66 :81-93
[9]  
Chung S, 2015, FASEB J, V29
[10]   Hypoxia and inflammatory bowel disease [J].
Cummins, Eoin P. ;
Crean, Daniel .
MICROBES AND INFECTION, 2017, 19 (03) :210-221