Human proteins that interact with RNA/DNA hybrids

被引：137

作者：

Wang, Isabel X. ^{[1
,2
]}

Grunseich, Christopher ^{[3
]}

Fox, Jennifer ^{[1
,2
]}

Burdick, Joshua ^{[1
,2
]}

Zhu, Zhengwei ^{[2
,4
]}

Ravazian, Niema ^{[1
]}

Hafner, Markus ^{[5
]}

Cheung, Vivian G. ^{[1
,2
,4
]}

机构：

[1] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[2] Univ Michigan, Life Sci Inst, Ann Arbor, MI 48109 USA

[3] NINDS, Neurogenet Branch, NIH, Bethesda, MD 20892 USA

[4] Univ Michigan, Dept Pediat, Ann Arbor, MI 48109 USA

[5] NIAMSD, Lab Muscle Stem Cells & Gene Regulat, Bethesda, MD 20892 USA

来源：

GENOME RESEARCH | 2018年 / 28卷 / 09期

关键词：

DNA-RNA HYBRIDS; R-LOOPS; BINDING PROTEIN; WIDE IDENTIFICATION; CRYSTAL-STRUCTURE; RIBONUCLEASE-H; TRANSCRIPTION; GENOME; COMPLEX; REVEALS;

D O I：

10.1101/gr.237362.118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA/DNA hybrids form when RNA hybridizes with its template DNA generating a three-stranded structure known as the R-loop. Knowledge of how they form and resolve, as well as their functional roles, is limited. Here, by pull-down assays followed by mass spectrometry, we identified 803 proteins that bind to RNA/DNA hybrids. Because these proteins were identified using in vitro assays, we confirmed that they bind to R-loops in vivo. They include proteins that are involved in a variety of functions, including most steps of RNA processing. The proteins are enriched for K homology (KH) and helicase domains. Among them, more than 300 proteins preferred binding to hybrids than double-stranded DNA. These proteins serve as starting points for mechanistic studies to elucidate what RNA/DNA hybrids regulate and how they are regulated.

引用

页码：1405 / 1414

页数：10

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