2D profiling of tumor chemotactic and molecular phenotype at single cell resolution using a SERS-microfluidic chip

被引:13
作者
Zhang, Yizhi [1 ,2 ]
Wu, Lei [1 ]
Yang, Kuo [1 ]
Zong, Shenfei [1 ]
Wang, Zhuyuan [1 ]
Cui, Yiping [1 ]
机构
[1] Southeast Univ, Adv Photon Ctr, Sch Elect Sci & Engn, Nanjing 210096, Peoples R China
[2] Nanjing Univ Aeronaut & Astronaut, Dept Biomed Engn, Nanjing 211106, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
gold@silver nanoparticles; surface enhanced Raman spectroscopy; microfluidic chip; single cell analysis; two-dimensional (2D) phenotype profiling; BREAST-CANCER; HETEROGENEITY; MIGRATION; PROGRESSION; METASTASIS;
D O I
10.1007/s12274-022-4100-5
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Emerging single-cell technologies create new opportunities for unraveling tumor heterogeneity. However, the development of high-content phenotyping platform is still at its infancy. Here, we develop a microfluidic chip for two-dimensional (2D) profiling of tumor chemotactic and molecular features at single cell resolution. Individual cells were captured by the triangular micropillar arrays in the cell-loading channel, facilitating downstream single-cell analysis. For 2D phenotyping, the chemotactic properties of tumor cells were visualized through cellular migratory behavior in microchannels, while their protein expression was profiled with multiplex surface enhanced Raman scattering (SERS) nanovectors, in which Raman reporter-embedded gold@silver core-shell nanoparticles (Au@Ag REPs) were modified with DNA aptamers targeting cellular surface proteins. As a proof of concept, breast cancer cells with diverse phenotypes were tested on the chip, demonstrating the capability of this platform for simultaneous chemotactic and molecular analysis. The chip is expected to provide a powerful tool for investigating tumor heterogeneity and promoting clinical precision medicine.
引用
收藏
页码:4357 / 4365
页数:9
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