Angstrom-scale chemical engineering: Multilabeled 2'-amino-LNA probes for nucleic acid detection in homogenous fluorescence assays

被引：0

作者：

Hrdlicka, PJ ^{[1
]}

Ravindra Babu, B ^{[1
]}

Sorensen, MD ^{[1
]}

Harrit, N ^{[1
]}

Wengel, J ^{[1
]}

机构：

[1] Univ So Denmark, Dept Chem, Nucl Acid Ctr, DK-5230 Odense, Denmark

来源：

Chemistry of Nucleic Acid Components | 2005年 / 7卷

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Development of sensitive probes for detection of nucleic acids in homogenous fluorescence assays is described. Incorporation of N2'-pyrene-functionalized 2'-amino-LNA monomers into DNA or RNA probe strands induces very bright fluorescence of duplexes formed between the probes and DNA/RNA complements. Large hybridization-induced increases in fluorescence intensity are obtained with probes containing two or more separated monomers.

引用

页码：27 / 28

页数：2

共 2 条

[1] Interstrand communication between 2′-N-(pyren-1-yl)methyl-2′-amino-LNA monomers in nucleic acid duplexes:: directional control and signalling of full complementarity [J].

Hrdlicka, PJ ;

Babu, BR ;

Sorensen, MD ;

Wengel, J .

CHEMICAL COMMUNICATIONS, 2004, (13) :1478-1479

[2] Functionalized LNA (locked nucleic acid):: high-affinity hybridization of oligonucleotides containing N-acylated and N-alkylated 2′-amino-LNA monomers [J].

Sorensen, MD ;

Petersen, M ;

Wengel, J .

CHEMICAL COMMUNICATIONS, 2003, (17) :2130-2131

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