Synthesis of Fluorescent Silica Nanoparticles and Their Applications as Fluorescence Probes

被引：22
作者：
Song, Xu [1 ]
Li, Fang [2 ]
Ma, Jingwei [3 ]
Jia, Nengqin [1 ]
Xu, Jianming [4 ]
Shen, Hebai [1 ]
机构：
[1] Shanghai Normal Univ, Dept Chem, Shanghai 200234, Peoples R China
[2] Tongji Univ, Sch Med, Shanghai Matern & Infant Hosp 1, Shanghai 200040, Peoples R China
[3] Shanghai BaiHuiShen Biosci & Tech Co Ltd, Shanghai 200030, Peoples R China
[4] Fudan Univ, Sch Med, Zhongshan Hosp, Shanghai 200030, Peoples R China
来源：
JOURNAL OF FLUORESCENCE | 2011年 / 21卷 / 03期
关键词：
Fluorescent silica nanoparticles; Luminescence; Fluorescent probe; Cellular imaging; QUANTUM DOTS; NANOSPHERES; PARTICLES; GROWTH; CELLS;
D O I：
10.1007/s10895-010-0799-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper presents the synthesis of organic dye molecules embedded silica nanoparticles by Stober method and their applications as fluorescence probes in cell imaging. By modifying the surface of fluorescent silica nanoparticles (FSNs) with amino, biologically functionalized and monodisperse FSNs can be obtained. In this work, FSNs were conjugated with monoclonal anti-Carcinoembryonic Antigen (anti-CEA) antibody via covalent binding. The antibody-conjugated FSNs can be used to label the SPCA-1 cells successfully, demonstrating that the application of FSNs as fluorescence probes in fluorescence imaging and bioassay would be feasible.
引用
收藏
页码：1205 / 1212
页数：8
相关论文
共 50 条
  • [21] Fluorescent proteins as efficient tools for evaluating the surface PEGylation of silica nanoparticles
    Zhang, Wei
    Ma, Minyan
    Zhang, Xiao-ai
    Zhang, Ze-yu
    Saleh, Sayed M.
    Wang, Xu-dong
    [J]. METHODS AND APPLICATIONS IN FLUORESCENCE, 2017, 5 (02):
  • [22] Fabrication of Fluorescent Silica Nanoparticles Hybridized with AIE Luminogens and Exploration of Their Applications as Nanobiosensors in Intracellular Imaging
    Faisal, Mahtab
    Hong, Yuning
    Liu, Jianzhao
    Yu, Yong
    Lam, Jacky W. Y.
    Qin, Anjun
    Lu, Ping
    Tang, Ben Zhong
    [J]. CHEMISTRY-A EUROPEAN JOURNAL, 2010, 16 (14) : 4266 - 4272
  • [23] Synthesis of Silica Nanoparticles Covered with Silver Beads
    Joksimovic, Rastko
    Altin, Burcu
    Mehta, Surinder K.
    Gradzielski, Michael
    [J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2013, 13 (10) : 6773 - 6781
  • [24] Fluorescent magnetic nanoparticles for biomedical applications
    Chekina, Nataliya
    Horak, Daniel
    Jendelova, Pavla
    Trchova, Miroslava
    Benes, Milan J.
    Hruby, Martin
    Herynek, Vit
    Turnovcova, Karolina
    Sykova, Eva
    [J]. JOURNAL OF MATERIALS CHEMISTRY, 2011, 21 (21) : 7630 - 7639
  • [25] Progresses and applications of protein fluorescent probes
    Chen Zhenzhen
    Zhang Ning
    Zhang Wenshen
    Tang Bo
    [J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2006, 34 (09) : 1341 - 1347
  • [26] Use of Fluorescent NIR Dyes in Silica Nanoparticles and as Enzyme Substrates in Bioanalytical Applications
    Patonay, Gabor
    Chapman, Gala
    Beckford, Garfield
    Henary, Maged
    Ellis, Holly
    [J]. REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS VI, 2014, 8956
  • [27] Novel functionalized ternary copolymer fluorescent nanoparticles: synthesis, fluorescent characteristics and protein immobilization
    Lu, Maolin
    Wu, Daocheng
    Guo, Na
    [J]. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2009, 20 (02) : 563 - 572
  • [28] Selective Selenol Fluorescent Probes: Design, Synthesis, Structural Determinants, and Biological Applications
    Zhang, Baoxin
    Ge, Chunpo
    Yao, Juan
    Liu, Yaping
    Xie, Huichen
    Fang, Jianguo
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (02) : 757 - 769
  • [29] A review of bioselenol-specific fluorescent probes: Synthesis, properties, and imaging applications
    Liu, Yuning
    Feng, Xiaohui
    Yu, Yanan
    Zhao, Qingyu
    Tang, Chaohua
    Zhang, Junmin
    [J]. ANALYTICA CHIMICA ACTA, 2020, 1110 (1110) : 141 - 150
  • [30] Design, Synthesis, Application and Research Progress of Fluorescent Probes
    Jiang, Xingxiu
    Yang, Ruizhu
    Lei, Xueli
    Xue, Shun
    Wang, Zhe
    Zhang, Jinyang
    Yan, Lan
    Xu, Zhiyi
    Chen, Zhengcheng
    Zou, Ping
    Wang, Guangtu
    [J]. JOURNAL OF FLUORESCENCE, 2024, 34 (03) : 965 - 975
12345