Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

被引：2

作者：

Zhang, Xuzhi ^{[1
]}

Ji, Xinming ^{[2
]}

Cui, Zhengguo ^{[1
]}

Yang, Bing ^{[1
]}

Huang, Jie ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

[2] Fudan Univ, State Key Lab ASIC & Syst, Dept Microelect, Shanghai 200433, Peoples R China

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2012年 / 33卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DNA chips; WSSV gene; Multi-channel electrochemical detection; Electroanalysis; Loop-mediated isothermal amplification; SYNDROME VIRUS WSSV; MODIFIED ELECTRODE; CARBON NANOTUBES; REAL-TIME; HYBRIDIZATION; BIOSENSORS; GOLD; SPOT; PCR; MICROCHIP;

D O I：

10.5012/bkcs.2012.33.1.69

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multi-channel chips had the potential for a high effective microdevice to recognize specific gene sequence for point-of-care applications.

引用

页码：69 / 75

页数：7

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