Highly Amino Acid Selective Hydrolysis of Myoglobin at Aspartate Residues as Promoted by Zirconium(IV)-Substituted Polyoxometalates

被引：94

作者：

Ly, Hong Giang T. ^{[1
]}

Absillis, Gregory ^{[1
]}

Janssens, Rik ^{[2
]}

Proost, Paul ^{[2
]}

Parac-Vogt, Tatjana N. ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, B-3001 Louvain, Belgium

[2] Katholieke Univ Leuven, Dept Microbiol & Immunol, Rega Inst, Lab Mol Immunol, B-3000 Louvain, Belgium

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 25期

关键词：

homogeneous catalysis; horse-heart myoglobin; hydrolysis; metalloproteases; polyoxometalates; KEGGIN-TYPE POLYOXOMETALATE; PEPTIDE-BOND HYDROLYSIS; HUMAN SERUM-ALBUMIN; NEUTRAL PH; SUBSTITUTED POLYOXOTUNGSTATES; REGIOSELECTIVE CLEAVAGE; COMPLEXES; PROTEINS; ZR; SPECTROSCOPY;

D O I：

10.1002/anie.201502006

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

SDS-PAGE/Edman degradation and HPLC MS/MS showed that zirconium(IV)-substituted Lindqvist-, Keggin-, and Wells-Dawson-type polyoxometalates (POMs) selectively hydrolyze the protein myoglobin at Asp X peptide bonds under mildly acidic and neutral conditions. This transformation is the first example of highly sequence selective protein hydrolysis by POMs, a novel class of protein-hydrolyzing agents. The selectivity is directed by Asp residues located on the surface of the protein and is further assisted by electrostatic interactions between the negatively charged POMs and positively charged surface patches in the vicinity of the cleavage site.

引用

页码：7391 / 7394

页数：4

共 24 条

[1] Peptide Bond Hydrolysis Catalyzed by the Wells-Dawson Zr(α2-P2W17O61)2 Polyoxometalate
Absillis, Gregory
Parac-Vogt, Tatjana N.
[J]. INORGANIC CHEMISTRY, 2012, 51 (18) : 9902 - 9910
[2] [Anonymous], ORGANIC CHEM DRUG DE
[3] Zirconium(IV)- and hafnium(IV)-containing polyoxometalates as oxidation precatalysts: Homogeneous catalytic epoxidation of cyclooctene by hydrogen peroxide
Aoto, Hiroki
Matsui, Keisuke
Sakai, Yoshitaka
Kuchizi, Teppei
Sekiya, Hiromi
Osada, Hironori
Yoshida, Takuya
Matsunaga, Satoshi
Nomiya, Kenji
[J]. JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL, 2014, 394 : 224 - 231
[4] Increased Lewis acidity in hafnium-substituted polyoxotungstates
Boglio, Cecile
Micoine, Kevin
Remy, Pauline
Hasenknopf, Bernold
Thorimbert, Serge
Lacote, Emmanuel
Malacria, Max
Afonso, Carlos
Tabet, Jean-Claude
[J]. CHEMISTRY-A EUROPEAN JOURNAL, 2007, 13 (19) : 5426 - 5432
[5] Buckingham DA, 2001, MET IONS BIOL SYST, V38, P43
[6] Molecular interactions between serum albumin proteins and Keggin type polyoxometalates studied using luminescence spectroscopy
Goovaerts, Vincent
Stroobants, Karen
Absillis, Gregory
Parac-Vogt, Tatjana N.
[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2013, 15 (42) : 18378 - 18387
[7] Major advances in the hydrolysis of peptides and proteins by metal ions and complexes
Grant, Kathryn B.
Kassai, Miki
[J]. CURRENT ORGANIC CHEMISTRY, 2006, 10 (09) : 1035 - 1049
[8] Synergistic Combination of Multi-ZrIV Cations and Lacunary Keggin Germanotungstates Leading to a Gigantic Zr24-Cluster-Substituted Polyoxometalate
Huang, Ling
Wang, Sa-Sa
Zhao, Jun-Wei
Cheng, Lin
Yang, Guo-Yu
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (21) : 7637 - 7642
[9] Unprecedented acceleration of zirconium(IV)-assisted peptide hydrolysis at neutral pH
Kassai, M
Ravi, RG
Shealy, SJ
Grant, KB
[J]. INORGANIC CHEMISTRY, 2004, 43 (20) : 6130 - 6132
[10] Tuning Zr(IV)-assisted peptide hydrolysis at near-neutral pH
Kassai, Miki
Grant, Kathryn B.
[J]. INORGANIC CHEMISTRY COMMUNICATIONS, 2008, 11 (05) : 521 - 525

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