Highly Amino Acid Selective Hydrolysis of Myoglobin at Aspartate Residues as Promoted by Zirconium(IV)-Substituted Polyoxometalates

被引：95

作者：

Ly, Hong Giang T. ^{[1
]}

Absillis, Gregory ^{[1
]}

Janssens, Rik ^{[2
]}

Proost, Paul ^{[2
]}

Parac-Vogt, Tatjana N. ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, B-3001 Louvain, Belgium

[2] Katholieke Univ Leuven, Dept Microbiol & Immunol, Rega Inst, Lab Mol Immunol, B-3000 Louvain, Belgium

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 25期

关键词：

homogeneous catalysis; horse-heart myoglobin; hydrolysis; metalloproteases; polyoxometalates; KEGGIN-TYPE POLYOXOMETALATE; PEPTIDE-BOND HYDROLYSIS; HUMAN SERUM-ALBUMIN; NEUTRAL PH; SUBSTITUTED POLYOXOTUNGSTATES; REGIOSELECTIVE CLEAVAGE; COMPLEXES; PROTEINS; ZR; SPECTROSCOPY;

D O I：

10.1002/anie.201502006

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

SDS-PAGE/Edman degradation and HPLC MS/MS showed that zirconium(IV)-substituted Lindqvist-, Keggin-, and Wells-Dawson-type polyoxometalates (POMs) selectively hydrolyze the protein myoglobin at Asp X peptide bonds under mildly acidic and neutral conditions. This transformation is the first example of highly sequence selective protein hydrolysis by POMs, a novel class of protein-hydrolyzing agents. The selectivity is directed by Asp residues located on the surface of the protein and is further assisted by electrostatic interactions between the negatively charged POMs and positively charged surface patches in the vicinity of the cleavage site.

引用

页码：7391 / 7394

页数：4

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