Influence of electroporation conditions on transfection of muscle fibers in vivo

被引：2

作者：

Ostapenko, OV ^{[1
]}

Boitsov, AS

Baranov, AN

Lesina, EA

Mikhailov, VM

Baranov, VS

机构：

[1] Ott Inst Obstet & Gynecol, St Petersburg 199034, Russia

[2] St Petersburg State Tech Univ, St Petersburg 195251, Russia

[3] Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia

来源：

RUSSIAN JOURNAL OF GENETICS | 2004年 / 40卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

D O I：

10.1023/B:RUGE.0000013446.86631.fd

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

This study is a survey of in vivo experiments on transfection of laboratory mouse muscle fibers by electoporation using an original device generating electric impulses. Transfection efficiency proved to depend on DNA dose and the number of electric impulses. It can be increased significantly by electroporation at varying pulse burst polarity. At both direct electrode application to muscles and electroporation through the skin, the muscle fiber transfection was more efficient under electroporation conditions much milder than those usually reported. The use of electroporation method for gene therapy of Duchenne muscular dystrophy is discussed.

引用

页码：33 / 39

页数：7

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[21] Stable transfection of MSCs by electroporation [J].

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[22] ELECTROPORATION TECHNIQUE OF DNA TRANSFECTION [J].

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[29] EVALUATION OF IN VIVO SKELETAL MUSCLE ELECTROPORATION AS A METHOD FOR THE STUDY OF SKELETAL MUSCLE ATROPHY [J].

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[30] Optimization of culture conditions to enhance transfection of human CD34+ cells by electroporation [J].

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