TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis

被引：608

作者：

Szemenyei, Heidi ^{[1
,2
]}

Hannon, Mike ^{[1
,2
]}

Long, Jeff A. ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

来源：

SCIENCE | 2008年 / 319卷 / 5868期

关键词：

D O I：

10.1126/science.1151461

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUXIN/ INDOLE- 3- ACETIC ACID ( AUX/ IAA) transcriptional repressors, freeing their binding partners, the AUXIN RESPONSE FACTOR ( ARF) proteins, which can activate transcription of auxin response genes. We show that TOPLESS ( TPL) can physically interact with IAA12/ BODENLOS ( IAA12/ BDL) through an ETHYLENE RESPONSE FACTOR ( ERF) - associated amphiphilic repression ( EAR) motif. TPL can repress transcription in vivo and is required for IAA12/ BDL repressive activity. In addition, tpl- 1 can suppress the patterning defects of the bdl- 1 mutant. Direct interaction between TPL and ARF5/ MONOPTEROS, which is regulated by IAA12/ BDL, results in a loss- of- function arf5/ mp phenotype. These observations show that TPL is a transcriptional co- repressor and further our understanding of how auxin regulates transcription during plant development.

引用

页码：1384 / 1386

页数：3

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