Imaging Immune and Metabolic Cells of Visceral Adipose Tissues with Multimodal Nonlinear Optical Microscopy

被引:15
作者
Urasaki, Yasuyo [1 ,2 ]
Johlfs, Mary G. [3 ,4 ]
Fiscus, Ronald R. [3 ,4 ]
Le, Thuc T. [1 ,2 ]
机构
[1] Desert Res Inst, Human Hlth & Environm Program, Las Vegas, NV USA
[2] Nevada Canc Inst, Dept Drug Dev, Las Vegas, NV USA
[3] Roseman Univ Hlth Sci, Ctr Diabet & Obes Prevent Treatment Res & Educ, Henderson, NV USA
[4] Roseman Univ Hlth Sci, Coll Pharm, Henderson, NV USA
来源
PLOS ONE | 2012年 / 7卷 / 06期
关键词
LIPID DROPLETS; OBESITY; INFLAMMATION; FLUORESCENCE; MACROPHAGES; LIPOLYSIS; PROTEINS; CANCER;
D O I
10.1371/journal.pone.0038418
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Visceral adipose tissue (VAT) inflammation is recognized as a mechanism by which obesity is associated with metabolic diseases. The communication between adipose tissue macrophages (ATMs) and adipocytes is important to understanding the interaction between immunity and energy metabolism and its roles in obesity-induced diseases. Yet visualizing adipocytes and macrophages in complex tissues is challenging to standard imaging methods. Here, we describe the use of a multimodal nonlinear optical (NLO) microscope to characterize the composition of VATs of lean and obese mice including adipocytes, macrophages, and collagen fibrils in a label-free manner. We show that lipid metabolism processes such as lipid droplet formation, lipid droplet microvesiculation, and free fatty acids trafficking can be dynamically monitored in macrophages and adipocytes. With its versatility, NLO microscopy should be a powerful imaging tool to complement molecular characterization of the immunity-metabolism interface.
引用
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页数:8
相关论文
共 37 条
  • [1] Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes
    Ariotti, Nicholas
    Murphy, Samantha
    Hamilton, Nicholas A.
    Wu, Lizhen
    Green, Kathryn
    Schieber, Nicole L.
    Li, Peng
    Martin, Sally
    Parton, Robert G.
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2012, 23 (10) : 1826 - 1837
  • [2] Integration of metabolism and inflammation by lipid-activated nuclear receptors
    Bensinger, Steven J.
    Tontonoz, Peter
    [J]. NATURE, 2008, 454 (7203) : 470 - 477
  • [3] Overweight, obesity and cancer: Epidemiological evidence and proposed mechanisms
    Calle, EE
    Kaaks, R
    [J]. NATURE REVIEWS CANCER, 2004, 4 (08) : 579 - 591
  • [4] Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms
    Campagnola, PJ
    Loew, LM
    [J]. NATURE BIOTECHNOLOGY, 2003, 21 (11) : 1356 - 1360
  • [5] Abdominal obesity and metabolic syndrome
    Despres, Jean-Pierre
    Lemieux, Isabelle
    [J]. NATURE, 2006, 444 (7121) : 881 - 887
  • [6] Regulation of the nitric oxide system in human adipose tissue
    Engeli, S
    Janke, J
    Gorzelniak, K
    Böhnke, J
    Ghose, N
    Lindschau, C
    Luft, FC
    Sharma, AM
    [J]. JOURNAL OF LIPID RESEARCH, 2004, 45 (09) : 1640 - 1648
  • [7] Fiscus RR, 2012, PROTEIN KIN IN PRESS, V68
  • [8] Fiscus RR, 2012, OVARIAN CANC BASIC S, P315
  • [9] Paracrine regulation of angiogenesis and adipocyte differentiation during in vivo adipogenesis
    Fukumura, D
    Ushiyama, A
    Duda, DG
    Xu, L
    Tam, J
    Chatterjee, VKK
    Garkavtsev, I
    Jain, RK
    [J]. CIRCULATION RESEARCH, 2003, 93 (09) : E88 - E97
  • [10] Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets
    Furuhashi, Masato
    Hotamisligil, Goekhan S.
    [J]. NATURE REVIEWS DRUG DISCOVERY, 2008, 7 (06) : 489 - 503