RNA interference inhibits replication of tick-borne encephalitis virus in vitro

被引：18

作者：

Achazi, Katharina ^{[1
]}

Patel, Pranav ^{[1
]}

Paliwal, Ravish ^{[1
]}

Radonic, Aleksandar ^{[1
]}

Niedrig, Matthias ^{[1
]}

Donoso-Mantke, Oliver ^{[1
]}

机构：

[1] Robert Koch Inst, Ctr Biol Secur ZBS 1 1, Nordufer 20, D-13353 Berlin, Germany

来源：

ANTIVIRAL RESEARCH | 2012年 / 93卷 / 01期

关键词：

Flavivirus; Tick-borne encephalitis virus; Tick-borne encephalitis; RNA interference; Small interfering RNA; Antiviral drug; YELLOW-FEVER VIRUS; REAL-TIME PCR; HEPATITIS-B-VIRUS; PASSIVE-IMMUNIZATION; NERVOUS-SYSTEM; SIRNA; DELIVERY; VIVO; ANTIBODIES; POTENT;

D O I：

10.1016/j.antiviral.2011.10.023

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Each year, up to 10,000 cases of infections with the flavivirus tick-borne encephalitis (TBE) virus that affect the central nervous system are reported in Europe and Asia. Due to the potentially severe adverse effects of post-exposure prophylaxis with TBE virus hyperimmunoglobulin, TBE can currently only be treated symptomatically. An RNA interference (RNAi) approach to inhibit TBE virus replication was therefore developed. In this study we demonstrate for the first time that small interfering RNAs (siRNAs) targeted at the TBE virus genome reduce the quantity of infectious TBE virus particles, TBE virus genome, and TBE virus protein in vitro by up to 85%. The 50% inhibitory dose (DI50) of the shRNA plasmid was only 0.05 mu g/ml. As RNAi-based therapeutics for other diseases are already being evaluated in phases II and Ill clinical trials, it is possible that RNAi could become valuable tool for controlling TBE virus infection. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：94 / 100

页数：7

共 57 条

[1] Detection and differentiation of tick-borne encephalitis virus subtypes by a reverse transcription quantitative real-time PCR and pyrosequencing [J].