Azadirachta indica exhibits chemopreventive action against hepatic cancer: Studies on associated histopathological and ultrastructural changes

被引:24
作者
Bharati, Sanjay [1 ]
Rishi, Praveen [2 ]
Koul, Ashwani [1 ]
机构
[1] Punjab Univ, Dept Biophys, Chandigarh 160014, India
[2] Punjab Univ, Dept Microbiol, Chandigarh 160014, India
关键词
hepatocellular carcinoma; transmission electron microscopy; scanning electron microscopy; BUCCAL POUCH CARCINOGENESIS; HEPATOCELLULAR-CARCINOMA; SKIN CARCINOGENESIS; N-NITROSAMINES; MODULATION; FLAVONOIDS; TOXICITY;
D O I
10.1002/jemt.21095
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The present study was designed to evaluate the anticarcinogenic potential of Azadirachta indica against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis. Further, the associated histopathological and ultrastructural changes were also analyzed. Hepatic cancer model was developed by the intraperitoneal administration of NDEA to mice at weekly intervals, in successive increasing doses, for a period of 8 weeks. Aqueous A. indica leaf extract (AAILE) was administered orally at a dosage of 100 mu g/g body weight thrice a week till termination of the study. A relationship between histopathological grading and chemopreventive effect of A. indica had been established at various stages of carcinogenesis. Anticancer activity of A. indica was evaluated in terms of tumor incidence, tumor multiplicity, and survival rate. A significant reduction in tumor incidence (33%), tumor multiplicity (42%), and increase in survival (34%) was observed upon administration of AAILE to NDEA-abused mice. Transmission and scanning electron microscopic investigations showed severe alterations in organelle organization, cellular arrangement, degree of differentiation, cellular metabolism, and morphology of the hepatocytes. These changes appeared to be distinctly delayed upon AAILE supplementation. The results suggest A. indica may have anticancer potential against NDEA-induced hepatic cancer. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc.
引用
收藏
页码:586 / 595
页数:10
相关论文
共 36 条
[1]   Azadirachta indica Exerts Chemopreventive Action Against Murine Skin Cancer: Studies on Histopathological, Ultrastructural Changes and Modulation of NF-κB, AP-1, and STAT1 [J].
Arora, N. ;
Bansal, M. P. ;
Koul, A. .
ONCOLOGY RESEARCH, 2011, 19 (05) :179-191
[2]   Chemopreventive Activity of Azadirachta indica on Two-stage Skin Carcinogenesis in Murine Model [J].
Arora, N. ;
Koul, A. ;
Bansal, M. P. .
PHYTOTHERAPY RESEARCH, 2011, 25 (03) :408-416
[3]   Azadirachta indica (neem): a plant of multiple biological and pharmacological activities [J].
Atawodi, Sunday E. ;
Atawodi, Joy C. .
PHYTOCHEMISTRY REVIEWS, 2009, 8 (03) :601-620
[4]   RELEVANCE OF NITROSAMINES TO HUMAN CANCER [J].
BARTSCH, H ;
MONTESANO, R .
CARCINOGENESIS, 1984, 5 (11) :1381-1393
[5]  
Biswas K, 2002, CURR SCI INDIA, V82, P1336
[6]   The determination of N-nitrosamines in food [J].
Crews, Colin .
QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS, 2010, 2 (01) :2-12
[7]  
Gangar SC, 2008, J ENVIRON PATHOL TOX, V27, P219
[8]  
Gangar SC, 2007, INDIAN J BIOCHEM BIO, V44, P209
[9]  
Guido I, 2010, IFMBE P, V25, P21
[10]  
Ho CL, 2003, J NUCL MED, V44, P213