An Amperometric Sensor for Nanomolar Detection of Hydrogen Peroxide Based on Encapsulation of Horseradish Peroxidase in Thymol Blue-Ormosil Composite

被引：8
作者：
Tiwari, Ida [1 ]
Singh, Manorama [1 ]
Tripathi, V. S. [2 ]
Lakshminarayana, G. [2 ]
Nogami, Masayuki [2 ]
机构：
[1] Banaras Hindu Univ, Fac Sci, Dept Chem, Varanasi 221005, Uttar Pradesh, India
[2] Nagoya Inst Technol Gokiso Cho, Showa Ku, Nagoya, Aichi 4668555, Japan
来源：
SENSOR LETTERS | 2011年 / 9卷 / 04期
关键词：
Amperometric Biosensor; Thymol Blue; Indium-Tin Oxide Electrode; Hydrogen Peroxide; Horseradish Peroxidase; Ormosil; Composite; GLASSY-CARBON ELECTRODE; GOLD NANOPARTICLES; OXIDE ELECTRODE; BIOSENSOR; IMMOBILIZATION; ENZYME; CATECHOLAMINE; FABRICATION; MEMBRANE; MEDIATOR;
D O I：
10.1166/sl.2011.1678
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An amperometric biosensor based on horseradish peroxidase and thymol blue-organically modified sol-gel glass composite has been fabricated for the determination of H2O2. We are for the first time reporting electrochemistry of thymol blue encapsulated within organically modified sol-gel glass. The biosensor has been characterized by electrochemical and amperometric measurements. The biosensor shows fast response with minimum interference. Under the optimized experimental conditions, H2O2 could be determined in a linear calibration range from 1.0 x 10(-8) M to 1.2 x 10(-3) M with a correlation coefficient of 0.998, sensitivity of 23.74 mu A/mM and a detection limit of 1 nM at S/N ratio = 3.
引用
收藏
页码：1323 / 1330
页数：8
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