Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector

被引：27

作者：

Cerovska, Noemi ^{[1
]}

Hoffmeisterova, Hana ^{[1
]}

Pecenkova, Tamara ^{[1
]}

Moravec, Tomas ^{[1
]}

Synkova, Helena ^{[1
]}

Plchova, Helena ^{[1
]}

Veleminsky, Jiri ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Expt Bot VVI, CZ-16000 Prague 6, Czech Republic

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2008年 / 58卷 / 01期

关键词：

epitopes expression; Human papillomavirus; transient expression; potyvirus-like particles; synergistic infection;

D O I：

10.1016/j.pep.2007.09.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The optimized expression of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. To increase the level of expressed protein the transgenic Nicotiana benthamiana plants expressing Potato virus A HC-Pro gene and transgenic Nicotiana tabacum, cv. Petit Havana SR1 carrying Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and Potato virus Y-o (PVYO) increased the expression of L2ACPE7 in N. tabacum and in transgenic N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：154 / 161

页数：8

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