Amperometric glucose biosensor based on in situ electropolymerized polyaniline/poly(acrylonitrile-co-acrylic acid) composite film

被引：55

作者：

Shan, Dan ^{[1
]}

Wang, Shanxia ^{[1
]}

He, Yuanyuan ^{[1
]}

Xue, Huaiguo ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Peoples R China

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 02期

基金：

中国国家自然科学基金;

关键词：

glucose biosensor; in situ electropolymerization; poly(acrylonitrile-co-acrylic acid); selectivity; porous film;

D O I：

10.1016/j.msec.2006.12.003

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A new type of in situ electropolymerization was used for electrochemical biosensor design. The biologic film was prepared by in situ electropolymerization of aniline into microporous poly(acrylonitrile-co-acrylic acid)-coated platinum electrode in the presence of glucose oxidase. The results of EIS and SEM indicated the successful immobilization for enzyme in the composite polymer film. The novel glucose biosensor exhibited good selectivity and operational stability, which showed no apparent loss of activity after 40 consecutive measurements and intermittent usage for 45 days with storage in a phosphate buffer solution at 4 degrees C. In addition, optimization of the biosensor construction as well as effects of applied potential, pH value of solution, temperature and common interfering compounds on the amperometric response of the sensor were investigated and discussed. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：213 / 217

页数：5

共 25 条

[1]

BRIGHT HJ, 1969, J BIOL CHEM, V244, P3625

[2] PLASTICIZED POLY(VINYL CHLORIDE) AS A PERMSELECTIVE BARRIER MEMBRANE FOR HIGH-SELECTIVITY AMPEROMETRIC SENSORS AND BIOSENSORS [J].

CHRISTIE, IM ;

TRELOAR, PH ;

VADGAMA, P .

ANALYTICA CHIMICA ACTA, 1992, 269 (01) :65-73

[3] Biosensors based on immobilization of biomolecules by electrogenerated polymer films -: New perspectives [J].

Cosnier, S .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2000, 89 (2-3) :127-138

[4] A new interference-free lysine biosensor using a non-conducting polymer film [J].

Curulli, A ;

Kelly, S ;

O'Sullivan, C ;

Guilbault, GG ;

Palleschi, G .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (12) :1245-1250

[5] Improvement of biosensor performances for nitrate determination using a new hydrophilic poly (pyrrole-viologen) film [J].

Da Silva, S ;

Shan, D ;

Cosnier, S .

SENSORS AND ACTUATORS B-CHEMICAL, 2004, 103 (1-2) :397-402

[6] Development of a biosensor for endocrine disrupting compounds based on tyrosinase entrapped within a poly(thionine) film [J].

Dempsey, E ;

Diamond, D ;

Collier, A .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (02) :367-377

[7] Application of conducting polymers to biosensors [J].

Gerard, M ;

Chaubey, A ;

Malhotra, BD .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (05) :345-359

[8]

Kesting R. E., 1985, SYNTHETIC POLYMERIC

[9] MOLECULAR IMPRINTING OF THEOPHYLLINE IN ACRYLONITRILE-ACRYLIC ACID COPOLYMER MEMBRANE [J].

KOBAYASHI, T ;

WANG, HY ;

FUJII, N .

CHEMISTRY LETTERS, 1995, (10) :927-928

[10] The selectivity of electrosynthesised polymer membranes depends on the electrode dimensions: implications for biosensor applications [J].

McMahon, CP ;

Killoran, SJ ;

Kirwan, SM ;

O'Neill, RD .

CHEMICAL COMMUNICATIONS, 2004, (18) :2128-2130

← 1 2 3 →