Development and validation of a simple high-performance liquid chromatography analytical method for simultaneous determination of phytosterols, cholesterol and squalene in parenteral lipid emulsions

被引：11

作者：

Novak, Ana ^{[1
]}

Gutierrez-Zamora, Merce ^{[2
]}

Domenech, Lluis ^{[2
]}

Sune-Negre, Josep M. ^{[1
,3
]}

Minarro, Montserrat ^{[1
,3
]}

Garcia-Montoya, Encarna ^{[1
,3
]}

Llop, Josep M. ^{[1
,3
]}

Tico, Josep R. ^{[1
,3
]}

Perez-Lozano, Pilar ^{[1
,3
]}

机构：

[1] Univ Barcelona, Fac Pharm & Food Sci, Dept Pharm & Pharmaceut Technol & Phys Chem, Barcelona, Spain

[2] Univ Barcelona, Fac Pharm & Food Sci, Serv Dev Med, Barcelona, Spain

[3] Bellvitge Biomed Res Inst IDIBELL, Pharmacotherapy Pharmacogenet & Pharmaceut Techno, Av Granvia de lHospitallet 199-203, Lhospitalet De Llobregat 08090, Spain

来源：

BIOMEDICAL CHROMATOGRAPHY | 2018年 / 32卷 / 02期

关键词：

cholesterol; high-performance liquid chromatography; phytosterol; squalene; validation; CONJUGATED PHYTOSTEROLS; VEGETABLE-OILS; HPLC; TOCOPHEROLS; STEROLS; FOODS; PLASMA; MS;

D O I：

10.1002/bmc.4084

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple analytical method for simultaneous determination of phytosterols, cholesterol and squalene in lipid emulsions was developed owing to increased interest in their clinical effects. Method development was based on commonly used stationary (C-18, C-8 and phenyl) and mobile phases (mixtures of acetonitrile, methanol and water) under isocratic conditions. Differences in stationary phases resulted in peak overlapping or coelution of different peaks. The best separation of all analyzed compounds was achieved on Zorbax Eclipse XDB C-8 (150x4.6mm, 5m; Agilent) and ACN-H2O-MeOH, 80:19.5:0.5 (v/v/v). In order to achieve a shorter time of analysis, the method was further optimized and gradient separation was established. The optimized analytical method was validated and tested for routine use in lipid emulsion analyses.

引用

页数：8

共 26 条

[1] Chromatographic analysis of plant sterols in foods and vegetable oils [J].

Abidi, SL .

JOURNAL OF CHROMATOGRAPHY A, 2001, 935 (1-2) :173-201

[2] An improved RP-HPLC method for simultaneous analyses of squalene and cholesterol especially in aquatic foods [J].

Bavisetty, Charan Bindu ;

Narayan, Bhaskar .

JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2015, 52 (09) :6083-6089

[3] Concept of sequential analysis of free and conjugated phytosterols in different plant matrices [J].

Breinhölder, P ;

Mosca, L ;

Lindner, W .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 777 (1-2) :67-82

[4]

DESJONG A, 2003, THE JOURNAL OF NUTRI, V14, P362, DOI DOI 10.1016/S0955-2863(03)00002-0

[5]

Duelund L., 2012, DETERMINATION STEROL

[6] Rapid measurement of phytosterols in fortified food using gas chromatography with flame ionization detection [J].

Duong, Samantha ;

Strobel, Norbert ;

Buddhadasa, Saman ;

Stockham, Katherine ;

Auldist, Martin ;

Wales, Bill ;

Orbell, John ;

Cran, Marlene .

FOOD CHEMISTRY, 2016, 211 :570-576

[7] Direct saponification preparation and analysis of free and conjugated phytosterols in sugarcane (Saccharum officinarum L.) by reversed-phase high-performance liquid chromatography [J].

Feng, Simin ;

Liu, Songbai ;

Luo, Zisheng ;

Tang, Kaichen .

FOOD CHEMISTRY, 2015, 181 :9-14

[8] Phytosterols: Applications and recovery methods [J].

Fernandes, P. ;

Cabral, J. M. S. .

BIORESOURCE TECHNOLOGY, 2007, 98 (12) :2335-2350

[9] Parenteral Lipid Emulsions in Guinea Pigs Differentially Influence Plasma and Tissue Levels of Fatty Acids, Squalene, Cholesterol, and Phytosterols [J].

Harvey, Kevin ;

Xu, Zhidong ;

Walker, Candace ;

Pavlina, Thomas ;

McGrath, Sheila ;

Zaloga, Gary ;

Siddiqui, Rafat .

LIPIDS, 2014, 49 (08) :777-793

[10] Dietary phytosterols as cholesterol-lowering agents in humans [J].

Jones, PJH ;

MacDougall, DE ;

Ntanios, F ;

Vanstone, CA .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1997, 75 (03) :217-227

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