Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

被引:11
作者
Balkema-Buschmann, Anne [1 ]
Rissmann, Melanie [1 ]
Kley, Nils [1 ]
Ulrich, Reiner [2 ]
Eiden, Martin [1 ]
Groschup, Martin H. [1 ]
机构
[1] Friedrich Loeffler Inst, Inst Novel & Emerging Infect Dis, D-17493 Greifswald, Germany
[2] Friedrich Loeffler Inst, Dept Expt Anim Facil & Biorisk Management, Sudufer 10, D-17493 Greifswald, Germany
来源
VIRUSES-BASEL | 2018年 / 10卷 / 12期
关键词
Rift Valley fever phlebovirus; MP-12 vaccine strain; virus reservoir; Rousettus aegyptiacus bat; RESERVOIR HOSTS; VIRUS; IMMUNOGENICITY; PATHOGENICITY; SAFETY;
D O I
10.3390/v10120681
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rift Valley fever phlebovirus (RVFV), the causative agent of an emerging zoonotic disease in Africa and Arabia, can infect a variety of species, predominantly ruminants, camelids, and humans. While clinical symptoms are mostly absent in adult ruminants and camelids, RVFV infection may lead to a serious, sometimes fatal disease in humans. Virus transmissions between individuals and between species mainly occur through mosquito bites, but direct or even indirect contact with infectious materials may also result in infection. Although the main reservoir of the virus is not yet identified, small mammals such as rodents and bats may act as amplifying hosts. We therefore inoculated Rousettus aegyptiacus fruit bats that are abundant in northern Africa with the vaccine strain MP-12, in order to elucidate the general competence of this species for virus propagation and transmission. We were able to detect the RVFV genome in the spleen of each of these animals, and re-isolated the virus from the spleen and liver of some animals. Moreover, we were able to identify the Gc RVFV surface antigen in mild subacute multifocal necrotizing hepatic lesions of one bat which was sacrificed 7 days post exposure. These findings demonstrate that Rousettus aegyptiacus fruit bats can propagate RVFV.
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页数:11
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