Suppressor of Hairless activates Brachyury expression in the Ciona embryo

被引:72
作者
Corbo, JC [1 ]
Fujiwara, S [1 ]
Levine, M [1 ]
Di Gregorio, A [1 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Div Genet, Berkeley, CA 94720 USA
关键词
Ciona intestinalis; Suppressor of Hairless; notochord; Brachyury;
D O I
10.1006/dbio.1998.9067
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Ciona Brachyury gene (Ci-Bra) is regulated, in part, by a 434-bp enhancer that mediates restricted expression in the notochord. Here we present evidence that a Ciona Suppressor of Hairless {Ci-Su(H)} protein functions as an activator of this enhancer. Point mutations that reduce the binding of a GST/Ci-Su(H) fusion protein in vitro diminish the expression of mutagenized Ci-Bra/lacZ transgenes in electroporated embryos. Overexpression of a Ci-Su(H) fusion protein containing the Drosophila Hairy repression domain interferes with notochord differentiation, producing mutant tadpoles with shortened tails. Expression of a constitutively activated Xotch receptor in the notochord, endoderm, and CNS also alters tail morphogenesis. These results suggest that a Notch-Su(H) pathway might participate in notochord differentiation in Ciona. (C) 1998 Academic Press.
引用
收藏
页码:358 / 368
页数:11
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