Development and In-House Validation of a Real-Time Polymerase Chain Reaction for the Detection of Listeria monocytogenes in Meat

被引：1
作者：
Reyes, Camilo [1 ]
Linares, Luciano H. [2 ]
Moredo, Fabiana [3 ]
Liron, Juan P. [1 ]
Brusa, Victoria [1 ,2 ]
Londero, Alejandra [1 ]
Galli, Lucia [1 ]
Oteiza, Juan M. [4 ]
Costa, Magdalena [1 ]
Leotta, Gerardo A. [1 ]
机构：
[1] UNLP, IGEVET Inst Genet Vet Ing Fernando N Dulout, CONICET, Fac Ciencias Vet, Ave 60 & 118,CC 296, RA-1900 La Plata, Buenos Aires, Argentina
[2] Univ Nacl La Plata, Lab Microbiol Alimentos, Fac Ciencias Vet, La Plata, Buenos Aires, Argentina
[3] UNLP, Catedra Microbiol, Fac Ciencias Vet, La Plata, Buenos Aires, Argentina
[4] Consejo Nacl Invest Cient & Tecn, Lab Microbiol Alimentos, Ctr Invest & Asistencia Tecn Ind CIATI AC, Neuquen, Argentina
来源：
FOODBORNE PATHOGENS AND DISEASE | 2018年 / 15卷 / 01期
关键词：
Listeria; detection; RT-PCR; validation; meat; PCR;
D O I：
10.1089/fpd.2017.2321
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Listeriosis is a foodborne disease caused by Listeria monocytogenes. The aims of this work were to develop and validate an in-house real-time polymerase chain reaction (RT-PCR) for the detection of L. monocytogenes, and to determine its prevalence in raw ground beef samples from 53 butcheries that also sell ready-to-eat foods. One set of primers and one hydrolysis probe were designed for hly gene detection and then challenged with pure strains. The detection was successful for all L. monocytogenes strains analyzed and negative for all non-L. monocytogenes strains (detection limit, 10 colony forming unit [CFU]/mL). Inclusivity, exclusivity, and analytical accuracy were 100%. L. monocytogenes was detected in 41.5% of raw ground beef samples from the 53 butcheries analyzed. This RT-PCR may be a valuable method for rapid detection of L. monocytogenes in meat.
引用
收藏
页码：55 / 57
页数：3
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