Lomefloxacin Induces Oxidative Stress and Apoptosis in COLO829 Melanoma Cells

被引:35
作者
Beberok, Artur [1 ]
Wrzesniok, Dorota [1 ]
Szlachta, Martyna [1 ]
Rok, Jakub [1 ]
Rzepka, Zuzanna [1 ]
Respondek, Michalina [1 ]
Buszman, Ewa [1 ]
机构
[1] Med Univ Silesia, Sch Pharm, Div Lab Med, Dept Pharmaceut Chem, Jagiellonska 4, PL-41200 Sosnowiec, Poland
关键词
lomefloxacin; melanoma; oxidative stress; DNA fragmentation; apoptosis; CYCLE ARREST; IN-VITRO; FLUOROQUINOLONES; CIPROFLOXACIN; TOPOISOMERASE; GLUTATHIONE; MECHANISM; LEUKEMIA; THERAPY; GROWTH;
D O I
10.3390/ijms18102194
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although some fluoroquinolones have been found to exert anti-tumor activity, studies on the effect of these drugs on melanoma cells are relatively rare. The aim of this study was to examine the effect of lomefloxacin on cell viability, reactive oxygen species production, redox balance, cell cycle distribution, DNA fragmentation, and apoptosis in COLO829 melanoma cells. Lomefloxacin decreases the cell viability in a dose- and time-dependent manner. For COLO829 cells treated with the drug for 24, 48, and 72 h, the values of IC50 were found to be 0.51, 0.33, and 0.25 mmol/L, respectively. The analyzed drug also altered the redox signaling pathways, as shown by intracellular reactive oxygen species overproduction and endogeneous glutathione depletion. After lomefloxacin treatment, the cells were arrested in S- and G2/M-phase, suggesting a mechanism related to topoisomerase II inhibition. DNA fragmentation was observed when the cells were exposed to increasing lomefloxacin concentrations and a prolongation of incubation time. Moreover, it was demonstrated that the drug induced mitochondrial membrane breakdown as an early hallmark of apoptosis. The obtained results provide a strong molecular basis for the pharmacologic effect underlying the potential use of lomefloxacin as a valuable agent for the treatment of melanoma in vivo.
引用
收藏
页数:18
相关论文
共 50 条
  • [41] Capsaicin induces apoptosis and autophagy in human melanoma cells
    Chu, Haihan
    Li, Meng
    Wang, Xiuchun
    ONCOLOGY LETTERS, 2019, 17 (06) : 4827 - 4834
  • [42] Flubendazole induces mitotic catastrophe and apoptosis in melanoma cells
    Canova, K.
    Rozkydalova, L.
    Vokurkova, D.
    Rudolf, E.
    TOXICOLOGY IN VITRO, 2018, 46 : 313 - 322
  • [43] Naringenin Induces Cellular Apoptosis in Melanoma Cells via Intracellular ROS Generation
    Fernando, Pincha devage sameera madushan
    Zhen, Ao xuan
    Piao, Mei jing
    Herath, Herath mudiyanselage udari lakmini
    Kang, Kyoung ah
    Yoon, Sang-pil
    Boo, Hye-jin
    Hyun, Chang lim
    Hyun, Jin won
    ANTICANCER RESEARCH, 2024, 44 (03) : 1079 - 1086
  • [44] 6-Shogaol as a Novel Thioredoxin Reductase Inhibitor Induces Oxidative-Stress-Mediated Apoptosis in HeLa Cells
    Peng, Shoujiao
    Yu, Shaopeng
    Zhang, Junmin
    Zhang, Jiange
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (05)
  • [45] Arsenic Trioxide Induces Apoptosis in Uveal Melanoma Cells Through the Mitochondrial Pathway
    Chen, Miao-Ju
    Yang, Pei-Yu
    Ye, Yi-Zhen
    Hu, Dan-Ning
    Chen, Ming-Feng
    AMERICAN JOURNAL OF CHINESE MEDICINE, 2010, 38 (06): : 1131 - 1142
  • [46] Sinularin induces oxidative stress-mediated G2/M arrest and apoptosis in oral cancer cells
    Chang, Yung-Ting
    Wu, Chang-Yi
    Tang, Jen-Yang
    Huang, Chiung-Yao
    Liaw, Chih-Chuang
    Wu, Shih-Hsiung
    Sheu, Jyh-Horng
    Chang, Hsueh-Wei
    ENVIRONMENTAL TOXICOLOGY, 2017, 32 (09) : 2124 - 2132
  • [47] Epoxy Stearic Acid, an Oxidative Product Derived from Oleic Acid, Induces Cytotoxicity, Oxidative Stress, and Apoptosis in HepG2 Cells
    Liu, Ying
    Cheng, Yajun
    Li, Jinwei
    Wang, Yuanpeng
    Liu, Yuanfa
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 66 (20) : 5237 - 5246
  • [48] Chromium(III) oxide nanoparticles induced remarkable oxidative stress and apoptosis on culture cells
    Horie, Masanori
    Nishio, Keiko
    Endoh, Shigehisa
    Kato, Haruhisa
    Fujita, Katsuhide
    Miyauchi, Arisa
    Nakamura, Ayako
    Kinugasa, Shinichi
    Yamamoto, Kazuhiro
    Niki, Etsuo
    Yoshida, Yasukazu
    Iwahashi, Hitoshi
    ENVIRONMENTAL TOXICOLOGY, 2013, 28 (02) : 61 - 75
  • [49] The mTOR promotes oxidative stress-induced apoptosis of mesangial cells in diabetic nephropathy
    Lu, Qian
    Zhou, Yuexian
    Hao, Meng
    Li, Chengcheng
    Wang, Jin
    Shu, Fanglin
    Du, Lei
    Zhu, Xia
    Zhang, Qiaoli
    Yin, Xiaoxing
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2018, 473 : 31 - 43
  • [50] Fructose induces mitochondrial dysfunction and triggers apoptosis in skeletal muscle cells by provoking oxidative stress
    Natasha Jaiswal
    Chandan K. Maurya
    Deepti Arha
    Deepa R. Avisetti
    Ayyappan Prathapan
    Palayyan S. Raj
    Kozhiparambil G. Raghu
    Shasi V. Kalivendi
    Akhilesh Kumar Tamrakar
    Apoptosis, 2015, 20 : 930 - 947