Although some fluoroquinolones have been found to exert anti-tumor activity, studies on the effect of these drugs on melanoma cells are relatively rare. The aim of this study was to examine the effect of lomefloxacin on cell viability, reactive oxygen species production, redox balance, cell cycle distribution, DNA fragmentation, and apoptosis in COLO829 melanoma cells. Lomefloxacin decreases the cell viability in a dose- and time-dependent manner. For COLO829 cells treated with the drug for 24, 48, and 72 h, the values of IC50 were found to be 0.51, 0.33, and 0.25 mmol/L, respectively. The analyzed drug also altered the redox signaling pathways, as shown by intracellular reactive oxygen species overproduction and endogeneous glutathione depletion. After lomefloxacin treatment, the cells were arrested in S- and G2/M-phase, suggesting a mechanism related to topoisomerase II inhibition. DNA fragmentation was observed when the cells were exposed to increasing lomefloxacin concentrations and a prolongation of incubation time. Moreover, it was demonstrated that the drug induced mitochondrial membrane breakdown as an early hallmark of apoptosis. The obtained results provide a strong molecular basis for the pharmacologic effect underlying the potential use of lomefloxacin as a valuable agent for the treatment of melanoma in vivo.
机构:
Norfolk State Univ, Dept Biol, Norfolk, VA USA
Norfolk State Univ, Ctr Biotechnol & Biomed Sci, Norfolk, VA USABangalore Univ, Dept Microbiol & Biotechnol, Bengaluru 560056, Karnataka, India
机构:
Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Wang, Xiao
Han, Chao
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Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Han, Chao
Cui, Yuqing
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Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Cui, Yuqing
Geng, Yumeng
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Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Geng, Yumeng
Wei, Yuanyuan
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Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Wei, Yuanyuan
Shi, Wanyu
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Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Hebei Prov Engn Technol Res Ctr Tradit Chinese Ve, Baoding 071001, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Shi, Wanyu
Bao, Yongzhan
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Hebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
Hebei Prov Engn Technol Res Ctr Tradit Chinese Ve, Baoding 071001, Peoples R ChinaHebei Agr Univ, Coll Tradit Chinese Vet Med, 2596 Le Kai South St, Baoding 071001, Hebei, Peoples R China
机构:
Nanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R ChinaNanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China
Xue, Chunyan
Chen, Yueqin
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Nanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China
New York Eye & Ear Infirm Mt Sinai, Tissue Culture Ctr, New York, NY 10003 USANanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China
Chen, Yueqin
Hu, Dan-Ning
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机构:
New York Eye & Ear Infirm Mt Sinai, Tissue Culture Ctr, New York, NY 10003 USANanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China
Hu, Dan-Ning
Iacob, Codrin
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New York Eye & Ear Infirm Mt Sinai, Dept Pathol, New York, NY 10003 USANanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China
Iacob, Codrin
Lu, Chengwei
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机构:
Jilin Univ, Dept Ophthalmol, Hosp 1, Changchun 130021, Jilin, Peoples R ChinaNanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China
Lu, Chengwei
Huang, Zhenping
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Nanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R ChinaNanjing Univ, Sch Med, Jinling Hosp, Dept Ophthalmol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China