Using 31P MAS NMR to monitor a gel phase thermal disorder transition in sphingomyelin/cholesterol bilayers

被引：7

作者：

Costello, Alison L. ^{[1
]}

Alam, Todd M. ^{[1
]}

机构：

[1] Sandia Natl Labs, Dept Nanostruct & Elect Mat, Albuquerque, NM 87185 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2008年 / 1778卷 / 01期

关键词：

solid-state nuclear magnetic resonance; sphingomyelin bilayer; magic-angle spinning; phase transition; cholesterol; gel phase;

D O I：

10.1016/j.bbamem.2007.08.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The impact of low cholesterol concentrations on an egg sphingomyelin bilayer is investigated using P-31 magic angle spinning (MAS) NMR spectroscopy. The magnitude of the isotropic P-31 MAS NMR line width is used to monitor the main gel to liquid crystalline phase transition, along with a unique gel phase pretransition. In addition, the P-31 chemical shift anisotropy (CSA) and spin-spin relaxation times (T-2), along with the effects of spinning speed, proton decoupling and magnetic field strength, are reported. The variation of this unique gel phase thermal pretransition with the inclusion of 5 through 21 mol% cholesterol is presented and discussed. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：97 / 104

页数：8