In situ detection of a single carotenoid crystal in a plant cell using Raman microspectroscopy

被引：28

作者：

Baranska, Malgorzata ^{[1
]}

Baranski, Rafal ^{[2
]}

Grzebelus, Ewa ^{[2
]}

Roman, Maciej ^{[1
]}

机构：

[1] Jagiellonian Univ, Fac Chem, PL-30060 Krakow, Poland

[2] Univ Agr Krakow, Dept Genet Plant Breeding & Seed Sci, PL-31425 Krakow, Poland

来源：

VIBRATIONAL SPECTROSCOPY | 2011年 / 56卷 / 02期

关键词：

FT-Raman; Mapping; Carotene; Subcellular analysis; Carrot; CARROT ROOT; ULTRASTRUCTURE; CHROMOPLASTS;

D O I：

10.1016/j.vibspec.2011.02.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

It is the first report of direct, in situ detection of carotenoids at the subcellular level by using Raman microspectroscopy. Single crystals sequestered in a carrot cell were measured using FT-Raman spectrometer equipped with a microscope and 40x objective. The observed characteristic bands centered at 1518 cm(-1) and 1156 cm(-1) proved the crystals were composed of carotenoids with beta-carotene being predominant. The obtained results show the potential of Raman microspectroscopy for identification and analysis of compounds localized in cytoplasm by taking measurements directly from a single plant cell. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：166 / 169

页数：4

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