Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells

被引：51

作者：

Seomun, Y ^{[1
]}

Kim, JA ^{[1
]}

Lee, EH ^{[1
]}

Joo, CK ^{[1
]}

机构：

[1] Catholic Univ Korea, Coll Med, Lab Ophthalmol & Visual Sci, Seocho Ku, Seoul 137701, South Korea

来源：

BIOCHEMICAL JOURNAL | 2001年 / 358卷

关键词：

cataract; epithelial-to-mesenchymal transformation; TGF-beta(1); transformation;

D O I：

10.1042/0264-6021:3580041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor-beta (TGF-beta) is known to be a causative factor in pathological fibrosis and the metastasis of cancer cells, through effects on molecules of the extracellular matrix (ECM). We evaluated the influence of TGF-beta (1), on the cnc expression of matrix metalloproteinase-2 (MMP-2) in lens epithelial cells (LECs). The results showed that TGF-beta (1), induced the expression of mRNA for MMP-2 in LECs. Subsequently, in order to examine the role of MMP-2,, we overexpressed MMP-2 in LECs by stable transfection. The MMP-2-overexpressing LECs showed typical indicators of a myofibroblast-like cell phenotype, such as multiple layers of cells, elongated morphology, and expression of a-smooth muscle actin. We also showed that an MMP inhibitor blocked the TGF-beta (1)-induced morphological change in LECs. These results demonstrate that MMP-2 plays a role in the transformation of LECs, which has implications for the pathological fibrosis of these cells.

引用

页码：41 / 48

页数：8

共 48 条

[1]

ANDLEY UP, 1994, INVEST OPHTH VIS SCI, V35, P3094

[2] Increased matrix metalloproteinase-2 activity induced by TGF-beta 1 in duct cells of human salivary gland is associated with the development of cyst formation in vivo [J].