Identification and Functional Analysis of Human CD56neg NK Cells by Flow Cytometry

被引：7

作者：

Orrantia, Ane ^{[1
]}

Terren, Inigo ^{[1
]}

Vitalle, Joana ^{[1
]}

Astarloa-Pando, Gabirel ^{[1
]}

Zenarruzabeitia, Olatz ^{[1
]}

Borrego, Francisco ^{[1
,2
]}

机构：

[1] Biocruces Bizkaia Hlth Res Inst, Immunopathol Grp, Baracaldo 48903, Spain

[2] Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 03期

关键词：

SUBSET; CMV;

D O I：

10.1016/j.xpro.2020.100149

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although scarce in the peripheral blood of healthy people, CD56neg NK cells are known to be expanded in some pathological conditions. However, studies on CD56neg NK cells had revealed contradictions, probably due to the lack of a spe-cific NK cell surface marker that helps to identify this subset. This protocol details the step-by-step procedure for the identification and functional analysis of CD56neg NK cells, providing an improved gating strategy for the selection of this intriguing population. For complete details on the use and execution of this protocol, please refer to Orrantia et al. (2020).

引用

页数：18

共 9 条

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