Inhibition of Candida albicans in vivo and in vitro by antimicrobial peptides chromogranin A-N12 through microRNA-155/suppressor of cytokine signaling 1 axis

被引：6
作者：
Li, Xiaohua [1 ,2 ]
Hu, Qun [3 ]
Lin, Qiong [1 ]
Luo, Jianxiong [1 ]
Xu, Junping [1 ]
Chen, Lifang [1 ]
Xu, Liyu [1 ]
Lin, Xin [1 ]
机构：
[1] Fujian Med Univ, Dept Pulm & Crit Care Med, Affiliated Fuzhou Hosp 1, Fuzhou, Fujian, Peoples R China
[2] 900 Hosp Joint Logist Team, Dept Pulm & Crit Care Med, Fuzhou, Fujian, Peoples R China
[3] Xia Men Univ, Dept Resp Crit Care & Sleep Med, Xiangan Hosp, Xiamen, Peoples R China
来源：
BIOENGINEERED | 2022年 / 13卷 / 02期
关键词：
Candida albicans; CGA-N12; SOCS1; antimicrobial peptides; miR-155; ANTIFUNGAL; ITRACONAZOLE; FLUCONAZOLE; EXPRESSION; CGA-N-12; EFFICACY;
D O I：
10.1080/21655979.2021.2017680
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Antimicrobial peptides (AMPs) have proven to inhibit a variety of pathogens. Chromogranin A-N12 (CGA-N12) is a kind of AMP, and it is characterized by stable structure, high anti-Candida activity, and good safety. However, it remains unclear whether CGA-N12 could effectively inhibit the growth of Candida albicans (C. albicans). Colony forming assays were used to measure minimal inhibitory concentration (MIC), minimal fungicidal concentration (MFC), and time-kill curve. Disseminated C. albicans rabbit model was established to investigate the influence of CGA-N12 on histological damage. The protein and mRNA levels of suppressor of cytokine signaling 1 (SOCS1) after treatment were investigated. The MIC and MFC of CGA-N12 against C. albicans was 6 mg/mL. CGA-N12 considerably inhibited germ tube formation of C. albicans. The fungal load in the tissues and inflammatory factors in the serum were suppressed by CGA-N12. CGA-N12 significantly reduced the histological changes caused by C. albicans, and the protein and mRNA levels of SOCS1 were markedly inhibited. The inhibition effect of CGA-N12 on C. albicans and significant improvement of histological damage by CGA-N12 through microRNA-155/SOCS1 axis were proved in this study. This study proposes a novel therapeutic strategy for the treatment and prevention of C. albicans.
引用
收藏
页码：2513 / 2524
页数：12
相关论文
共 34 条
[1]   Candida albicans in celiac disease: A wolf in sheep's clothing [J].
Aaron, Lerner ;
Torsten, Matthias .
AUTOIMMUNITY REVIEWS, 2020, 19 (09)
[2]   Efficacy of liposomal amphotericin B and anidulafungin using an antifungal lock technique (ALT) for catheter-related Candida albicsan and Candida glabrata infections in an experimental model [J].
Basas, Jana ;
Palau, Marta ;
Gomis, Xavier ;
Almirante, Benito ;
Gavalda, Joan .
PLOS ONE, 2019, 14 (02)
[3]   A New Era of Antibiotics: The Clinical Potential of Antimicrobial Peptides [J].
Browne, Katrina ;
Chakraborty, Sudip ;
Chen, Renxun ;
Willcox, Mark D. P. ;
Black, David StClair ;
Walsh, William R. ;
Kumar, Naresh .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (19) :1-23
[4]   The regulation of hyphae growth in Candida albicans [J].
Chen, Hui ;
Zhou, Xuedong ;
Ren, Biao ;
Cheng, Lei .
VIRULENCE, 2020, 11 (01) :337-348
[5]   Suppressors of cytokine signaling: Potential immune checkpoint molecules for cancer immunotherapy [J].
Chikuma, Shunsuke ;
Kanamori, Mitsuhiro ;
Mise-Omata, Setsuko ;
Yoshimura, Akihiko .
CANCER SCIENCE, 2017, 108 (04) :574-580
[6]   Candida albicans - Biology, molecular characterization, pathogenicity, and advances in diagnosis and control - An update [J].
Dadar, Maryam ;
Tiwari, Ruchi ;
Karthik, Kumaragurubaran ;
Chakraborty, Sandip ;
Shahali, Youcef ;
Dhama, Kuldeep .
MICROBIAL PATHOGENESIS, 2018, 117 :128-138
[7]   Antimicrobial Peptide rBPI21: A Translational Overview from Bench to Clinical Studies [J].
Domingues, Marco M. ;
Santos, Nuno C. ;
Castanho, Miguel A. R. B. .
CURRENT PROTEIN & PEPTIDE SCIENCE, 2012, 13 (07) :611-619
[8]   Experimental Candida albicans osteomyelitis: Microbiologic, antigenic, histologic, and 18FDG-PET-CT imaging characteristics in a newly established rabbit model [J].
Gamaletsou, Maria N. ;
Meletiadis, Joseph ;
Chatziioannou, Sofia ;
Panayiotides, Ioannis G. ;
Agadakos, Euthimios ;
Katsimpoulas, Michalis ;
Kostomitsopoulos, Nikolaos ;
Petraitis, Vidmantas ;
Walsh, Thomas J. ;
Sipsas, Nikolaos V. .
MEDICAL MYCOLOGY, 2019, 57 (08) :1011-1017
[9]   Chromogranin-A and its role in the pathogenesis of diabetes mellitus [J].
Herold, Zoltan ;
Doleschall, Marton ;
Kovesdi, Annamaria ;
Patocs, Attila ;
Somogyi, Aniko .
ENDOKRYNOLOGIA POLSKA, 2018, 69 (05) :598-610
[10]   Association of primary biliary cirrhosis with variants in the CLEC16A, SOCS1, SPIB and SIAE immunomodulatory genes [J].
Hirschfield, G. M. ;
Xie, G. ;
Lu, E. ;
Sun, Y. ;
Juran, B. D. ;
Chellappa, V. ;
Coltescu, C. ;
Mason, A. L. ;
Milkiewicz, P. ;
Myers, R. P. ;
Odin, J. A. ;
Luketic, V. A. ;
Bacon, B. ;
Bodenheimer, H. ;
Liakina, V. ;
Vincent, C. ;
Levy, C. ;
Pillai, S. ;
Lazaridis, K. N. ;
Amos, C. I. ;
Siminovitch, K. A. .
GENES AND IMMUNITY, 2012, 13 (04) :328-335
1234