Reutilisation of orange waste for production of lignocellulolytic enzymes by Pleurotus ostreatus (Jack:Fr)

In this work, orange waste as a substrate to produce hydrolytic and oxidative enzymes involved in the degradation of ligninocellulolytic materials, such as laccase (EC 1.10.3.2), manganese peroxidase (EC 1.11.1.14), xylanase (EC 3.2.1.8) and endo-1,4 glucanase (EC 3.2.1.4) by the Basidiomycete Pleurotus ostreatus was used. The fungus was able to grow in the orange waste at different moisture contents without any supplementation. The orange waste medium provided the conditions to produce high activities of important industrial enzymes, especially laccase (75 U.g(-1) substrate, after 15 days of cultivation) and Mn peroxidase (6.8 U.g(-1) substrate, after 30 days of cultivation). Keywords: industrial wastes; ligninolytic enzymes; cellulolytic enzymes; lacase.