Exosomes isolated from melatonin-stimulated mesenchymal stem cells improve kidney function by regulating inflammation and fibrosis in a chronic kidney disease mouse model

被引：44

作者：

Yea, Ji-Hye ^{[1
]}

Yoon, Yeo Min ^{[1
]}

Lee, Jun Hee ^{[2
,3
,4
,5
,6
]}

Yun, Chul Won ^{[1
]}

Lee, Sang Hun ^{[1
,7
,8
,9
]}

机构：

[1] Soonchunhyang Univ, Med Sci Res Inst, Seoul Hosp, Seoul, South Korea

[2] Dankook Univ, Inst Tissue Regenerat Engn, Cheonan, South Korea

[3] Dankook Univ, Dept Nanobiomed Sci, Cheonan, South Korea

[4] Dankook Univ, BK21 PLUS NBM Global Res Ctr Regenerat Med, Cheonan, South Korea

[5] Dankook Univ, Coll Dent, Dept Oral Anat, Cheonan, South Korea

[6] Dankook Univ, Cell & Matter Inst, Cheonan, South Korea

[7] Soonchunhyang Univ, Dept Biochem, Coll Med, Cheonan, South Korea

[8] Soonchunhyang Univ, Coll Med, Dept Biochem, BK21FOUR Project2, Cheonan, South Korea

[9] Stembio Ltd, Asan, South Korea

来源：

JOURNAL OF TISSUE ENGINEERING | 2021年 / 12卷

基金：

新加坡国家研究基金会;

关键词：

Chronic kidney disease; mesenchymal stem cell; exosome; inflammation; fibrosis; EXTRACELLULAR VESICLES; RENAL FIBROSIS; SURVIVAL; EXPRESSION; TARGETS; REPAIR;

D O I：

10.1177/20417314211059624

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Chronic kidney disease (CKD) is defined as structural and functional abnormalities of the kidney due to inflammation and fibrosis. We investigated the therapeutic effects of exosomes secreted by melatonin-stimulated mesenchymal stem cells (Exocue) on the functional recovery of the kidney in a CKD mouse model. Exocue upregulated gene expression of micro RNAs (miRNAs) associated with anti-inflammatory and anti-fibrotic effects. Exocue-treated groups exhibited low tumor necrosis factor-alpha and transforming growth factor-beta levels in serum and fibrosis inhibition in kidney tissues mediated through regulation of cell apoptosis and proliferation of fibrosis-related cells. Exocue treatment decreased the gene expression of CKD progression-related miRNAs. Moreover, the CKD severity was alleviated in the Exocue group via upregulation of aquaporin 2 and 5 levels and reduction of blood urea nitrogen and creatinine, resulting in functional recovery of the kidney. In conclusion, Exocue could be a novel therapeutic agent for treating CKD by regulating inflammation and fibrosis.

引用

页数：13

共 50 条

[1] Burden of Chronic Kidney Disease: An International Perspective [J].