The downy mildew resistance locus Pp523 is located on chromosome C8 of Brassica oleracea L.

被引:21
作者
Carlier, Jorge D. [1 ]
Alabaca, Claudia A. [1 ]
Coelho, Paula S. [2 ]
Monteiro, Antonio A. [3 ]
Leitao, Jose M. [1 ]
机构
[1] Univ Algarve, FCT, BioFIG, P-8005139 Faro, Portugal
[2] Inst Nacl Recursos Biol, P-2780505 Oeiras, Portugal
[3] Univ Tecn Lisboa, Inst Super Agron, P-1349017 Lisbon, Portugal
基金
美国国家科学基金会;
关键词
downy mildew; Brassica oleracea; genetic resistance; genetic map; chromosome location; ADULT-PLANT RESISTANCE; LINKAGE MAP; PERONOSPORA-PARASITICA; GENOME; NAPUS; IDENTIFICATION; MARKERS; MICROSATELLITES; ASSIGNMENT; ABUNDANCE;
D O I
10.1111/j.1439-0523.2011.01904.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
We have previously constructed a genetic map of Brassica oleracea L. containing the Pp523 locus that confers downy mildew resistance to adult plants. In this work, 44 SSR markers of reference for the Brassica C genome chromosomes were added to the map, allowing the nine major linkage groups to be assigned to the nine chromosomes of B. oleracea. Locus Pp523 was located on chromosome C8, and a locus determining flower colour was mapped to chromosome C3. In comparison with the first version of the map, the new map is denser and more compact. The available genomic information on B. oleracea was enriched with the chromosome location of two phenotypic traits and 421 DNA markers (RAPD, ISSR, AFLP, SCAR, BAC-end derived STS, SSR and other PCR markers). Conversely, the genomic information on B. oleracea chromosome C8 is being used as an additional tool for the map-based cloning of Pp523, the first gene for adult plant resistance to downy mildew precisely located to a specific chromosome of this crop species.
引用
收藏
页码:170 / 175
页数:6
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