Fast optically sectioned fluorescence HiLo endomicroscopy

被引:70
作者
Ford, Tim N. [1 ]
Lim, Daryl [1 ]
Mertz, Jerome [1 ]
机构
[1] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
关键词
endomicroscopy; fluorescence; optical sectioning; fiber-bundle imaging; double-shutter camera; graphics processing unit; chorioallantoic membrane; 2-PHOTON MICROSCOPE; FIBER BUNDLE; ENDOSCOPY;
D O I
10.1117/1.JBO.17.2.021105
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier demonstration of widefield optical sectioning through a flexible fiber bundle. The improved device features lateral and axial resolutions of 2.6 and 17 mu m, respectively, a net frame rate of 9.5 Hz obtained by real-time image processing with a graphics processing unit (GPU) and significantly reduced motion artifacts obtained by the use of a double-shutter camera. We demonstrate the performance of our system with optically sectioned images and videos of a fluorescently labeled chorioallantoic membrane (CAM) in the developing G. gallus embryo. HiLo endomicroscopy is a candidate technique for low-cost, high-speed clinical optical biopsies. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.2.021105]
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页数:7
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